ArticleApplied and environmental microbiology2007
Quantitative detection of Clostridium tyrobutyricum in milk by real-time PCR.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 57 citations in OpenAlex.
- Integrating Metabolic Modeling and Targeted Supplementation for the Rapid Detection ofMicroorganisms · 2026Article
- The effect of different milk pretreatment methods on microbiome community development during Herrgårds cheese production and ripening.PloS one · 2026Article
- Colorimetric Point-of-Care Detection ofBiosensors · 2021Article
- Advantages and limitations of potential methods for the analysis of bacteria in milk: a review.Journal of food science and technology · 2016Review
- Nucleic acid-based approaches to investigate microbial-related cheese quality defects.Frontiers in microbiology · 2013Article
- Real-time PCR detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum.Applied and environmental microbiology · 2012Article
- Mycoplasma agalactiae p40 Gene, a novel marker for diagnosis of contagious agalactia in sheep by real-time PCR: assessment of analytical performance and in-house validation using naturally contaminated milk samples.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a real-time PCR assay for the quantitative detection of Clostridium tyrobutyricum, which has been identified as the major causal agent of late blowing in cheese. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent in 40% of the reactions. The quantification was linear (R(2) > 0.9995) over a 5-log dynamic range, down to 10 genome equivalents, with a PCR efficiency of >0.946. With optimized detergent treatment and enzymatic pretreatment of the sample before centrifugation and nucleic acid extraction, the assay counted down to 300 C. tyrobutyricum spores, with a relative accuracy of 82.98 to 107.68, and detected as few as 25 spores in 25 ml of artificially contaminated raw or ultrahigh-temperature-treated whole milk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.