ArticleApplied and environmental microbiology2007
Trehalose biosynthesis in Rhizobium leguminosarum bv. trifolii and its role in desiccation tolerance.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 103 citations in OpenAlex.
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- Intracellular Protective Functions and Therapeutical Potential of Trehalose.Molecules (Basel, Switzerland) · 2024Review
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- Plant Beneficial Bacteria and Their Potential Applications in Vertical Farming Systems.Plants (Basel, Switzerland) · 2023Review
- Characterizing Effects of Microbial Biostimulants and Whole-Soil Inoculums for Native Plant Revegetation.Microorganisms · 2022Article
- Effects of trehalose and polyacrylate-based hydrogels on tomato growth under drought.AoB PLANTS · 2022Article
- The source and fate of Mycobacterium tuberculosis complex in wastewater and possible routes of transmission.BMC public health · 2022Article
- Maintaining osmotic balance in legume nodules.Journal of experimental botany · 2022Article
- Effects of rehydration on physiological and transcriptional responses of a water-stressed rhizobium.Journal of microbiology (Seoul, Korea) · 2022Article
- Programmable design of seed coating function induces water-stress tolerance in semi-arid regions.Nature food · 2021Article
- Enhancing the Efficiency of Soybean Inoculant for Nodulation under Multi-Environmental Stress Conditions.Polish journal of microbiology · 2021Article
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- Bioformulation of Silk-Based Coating to Preserve and DeliverFrontiers in plant science · 2021Article
- Rhizobial-Host Interactions and Symbiotic Nitrogen Fixation in Legume Crops Toward Agriculture Sustainability.Frontiers in microbiology · 2021Review
- Trehalose and bacterial virulence.Virulence · 2020Review
- Salt Stress Response of Sulfolobus acidocaldarius Involves Complex Trehalose Metabolism Utilizing a Novel Trehalose-6-Phosphate Synthase (TPS)/Trehalose-6-Phosphate Phosphatase (TPP) Pathway.Applied and environmental microbiology · 2020Article
- Characterization of genes responsive to osmotic and oxidative stresses of the sugarcane bacterial pathogen Leifsonia xyli subsp. xyli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Deciphering the Role of Trehalose in Tripartite Symbiosis Among Rhizobia, Arbuscular Mycorrhizal Fungi, and Legumes for Enhancing Abiotic Stress Tolerance in Crop Plants.Frontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobium leguminosarum bv. trifolii forms nitrogen-fixing root nodules on the pasture legume Trifolium repens, and T. repens seed is often coated with a compatible R. leguminosarum bv. trifolii strain prior to sowing. However, significant losses in bacterial viability occur during the seed-coating process and during storage of the coated seeds, most likely due to desiccation stress. The disaccharide trehalose is known to function as an osmoprotectant, and trehalose accumulation due to de novo biosynthesis is a common response to desiccation stress in bacteria. In this study we investigated the role of endogenous trehalose synthesis in desiccation tolerance in R. leguminosarum bv. trifolii strain NZP561. Strain NZP561 accumulated trehalose as it entered the stationary phase due to the combined actions of the TreYZ and OtsAB pathways. Mutants deficient in either pathway showed near-wild-type levels of trehalose accumulation, but double otsA treY mutants failed to accumulate any trehalose. The double mutants were more sensitive to the effects of drying, and their survival was impaired compared to that of the wild type when glass beads were coated with the organisms and stored at relative humidities of 5 and 32%. The otsA treY mutants were also less competitive for nodule occupancy. Gene expression studies showed that the otsA and treY genes were expressed constitutively and that expression was not influenced by the growth phase, suggesting that trehalose accumulation is controlled at the posttranscriptional level or by control of trehalose breakdown rates. Our results indicate that accumulated trehalose plays an important role in protecting R. leguminosarum bv. trifolii cells against desiccation stress and against stress encountered during nodulation.
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