ArticleApplied and environmental microbiology2007
DNA microarray-based identification of serogroups and virulence gene patterns of Escherichia coli isolates associated with porcine postweaning diarrhea and edema disease.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Comparative genomic analysis of 127 Escherichia coli strains isolated from domestic animals with diarrhea in China.BMC genomics · 2019Article
- Development and Assessment of a Diagnostic DNA Oligonucleotide Microarray for Detection and Typing of Meningitis-Associated Bacterial Species.High-throughput · 2018Article
- Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR.BMC microbiology · 2017Article
- Six Novel O Genotypes from Shiga Toxin-Producing Escherichia coli.Frontiers in microbiology · 2016Article
- Article
- Feed Fermentation with Reuteran- and Levan-Producing Lactobacillus reuteri Reduces Colonization of Weanling Pigs by Enterotoxigenic Escherichia coli.Applied and environmental microbiology · 2015Article
- Article
- Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia.BMC veterinary research · 2013Article
- Genetic analysis of the Cronobacter sakazakii O4 to O7 O-antigen gene clusters and development of a PCR assay for identification of all C. sakazakii O serotypes.Applied and environmental microbiology · 2012Article
- Use of a DNA microarray for detection and identification of bacterial pathogens associated with fishery products.Applied and environmental microbiology · 2011Article
- Development of an O-antigen serotyping scheme for Cronobacter sakazakii.Applied and environmental microbiology · 2011Article
- High-throughput microarray technology in diagnostics of enterobacteria based on genome-wide probe selection and regression analysis.BMC genomics · 2010Article
- Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli.BMC microbiology · 2010Article
- Nonstarch polysaccharides modulate bacterial microbiota, pathways for butyrate production, and abundance of pathogenic Escherichia coli in the pig gastrointestinal tract.Applied and environmental microbiology · 2010Article
- Development of a DNA microarray for detection and serotyping of enterotoxigenic Escherichia coli.Journal of clinical microbiology · 2010Article
- DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2009Article
- Evaluation of major types of Shiga toxin 2E-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans.Applied and environmental microbiology · 2008Article
- Empirical evaluation of a new method for calculating signal-to-noise ratio for microarray data analysis.Applied and environmental microbiology · 2008Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli strains causing postweaning diarrhea (PWD) and edema disease (ED) in pigs are limited to a number of serogroups, with O8, O45, O138, O139, O141, O147, O149, and O157 being the most commonly reported worldwide. In this study, a DNA microarray based on the O-antigen-specific genes of all 8 E. coli serogroups, as well as 11 genes encoding adhesion factors and exotoxins associated with PWD and ED, was developed for the identification of related serogroups and virulence gene patterns. The microarray method was tested against 186 E. coli and Shigella O-serogroup reference strains, 13 E. coli reference strains for virulence markers, 43 E. coli clinical isolates, and 12 strains of other bacterial species and shown to be highly specific with reproducible results. The detection sensitivity was 0.1 ng of genomic DNA or 10(3) CFU per 0.3 g of porcine feces in mock samples. Seventeen porcine feces samples from local hoggeries were examined using the microarray, and the result for one sample was verified by the conventional serotyping methods. This microarray can be readily used to screen for the presence of PWD- and ED-associated E. coli in porcine feces samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.