ArticleApplied and environmental microbiology2007
Identification of a gene cluster enabling Lactobacillus casei BL23 to utilize myo-inositol.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- NovelGut · 2026Article
- A culturomics approach reveals cross-feeding capacity of intestinal pig bacteria upon release of inositol from phytate.Microbiome · 2026Article
- Multi-omic stable isotope probing revealsISME communications · 2026Article
- Metabolism of inositol derivatives by the gut microbiome.Gut microbes · 2025Review
- Predicting rhizosphere-competence-related catabolic gene clusters in plant-associated bacteria with rhizoSMASH.Nature communications · 2025Article
- Integrating genomic evidence for an updated taxonomy of the bacterial genus Spiribacter.Scientific reports · 2024Article
- Phytate metabolism is mediated by microbial cross-feeding in the gut microbiota.Nature microbiology · 2024Article
- Nitrosative stress under microaerobic conditions triggers inositol metabolism in Pseudomonas extremaustralis.PloS one · 2024Article
- Assessment of beneficial effects and identification of host adaptation-associated genes of Ligilactobacillus salivarius isolated from badgers.BMC genomics · 2023Article
- Physiological, Biochemical, and Structural Bioinformatic Analysis of the Multiple Inositol Dehydrogenases from Corynebacterium glutamicum.Microbiology spectrum · 2022Article
- Metabolism in the Niche: a Large-Scale Genome-Based Survey Reveals Inositol Utilization To Be Widespread among Soil, Commensal, and Pathogenic Bacteria.Microbiology spectrum · 2022Review
- Comparative Genomics ofFrontiers in microbiology · 2022Article
- Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health.Nature communications · 2021Article
- Staphylococcal Protein A (mSphere · 2020Article
- Natural and engineered promoters for gene expression in Lactobacillus species.Applied microbiology and biotechnology · 2020Review
- Comparative genomics and functional analysis of a highly adhesive dairy Lactobacillus paracasei subsp. paracasei IBB3423 strain.Applied microbiology and biotechnology · 2019Article
- The genetic basis of 3-hydroxypropanoate metabolism inBiotechnology for biofuels · 2019Article
- Comparative genomic and metabolic analysis of three Lactobacillus paracasei cheese isolates reveals considerable genomic differences in strains from the same niche.BMC genomics · 2018Article
- Genetic Signatures of DairyFrontiers in microbiology · 2018Article
- Heat Survival and Phenotype Microarray Profiling of Salmonella Typhimurium Mutants.Current microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome analysis of Lactobacillus casei BL23 revealed that, compared to L. casei ATCC 334, it carries a 12.8-kb DNA insertion containing genes involved in the catabolism of the cyclic polyol myo-inositol (MI). Indeed, L. casei ATCC 334 does not ferment MI, whereas strain BL23 is able to utilize this carbon source. The inserted DNA consists of an iolR gene encoding a DeoR family transcriptional repressor and a divergently transcribed iolTABCDG1G2EJK operon, encoding a complete MI catabolic pathway, in which the iolK gene probably codes for a malonate semialdehyde decarboxylase. The presence of iolK suggests that L. casei has two alternative pathways for the metabolism of malonic semialdehyde: (i) the classical MI catabolic pathway in which IolA (malonate semialdehyde dehydrogenase) catalyzes the formation of acetyl-coenzyme A from malonic semialdehyde and (ii) the conversion of malonic semialdehyde to acetaldehyde catalyzed by the product of iolK. The function of the iol genes was verified by the disruption of iolA, iolT, and iolD, which provided MI-negative strains. By contrast, the disruption of iolK resulted in a strain with no obvious defect in MI utilization. Transcriptional analyses conducted with different mutant strains showed that the iolTABCDG1G2EJK cluster is regulated by substrate-specific induction mediated by the inactivation of the transcriptional repressor IolR and by carbon catabolite repression mediated by the catabolite control protein A (CcpA). This is the first example of an operon for MI utilization in lactic acid bacteria and illustrates the versatility of carbohydrate utilization in L. casei BL23.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.