ArticleApplied and environmental microbiology2007
The early response to acid shock in Lactobacillus reuteri involves the ClpL chaperone and a putative cell wall-altering esterase.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 130 citations in OpenAlex.
- Genomic, phenotypic, and clinical safety of Limosilactobacillus reuteri ATCC PTA 4659.Journal of industrial microbiology & biotechnology · 2023Trial
- Removal of antibiotic resistance gene-carrying plasmids from Lactobacillus reuteri ATCC 55730 and characterization of the resulting daughter strain, L. reuteri DSM 17938.Applied and environmental microbiology · 2008Trial
- Machine learning identifies key microbial signatures for Salmonella enteritidis colonization and persistence in broiler ceca.Poultry science · 2026Article
- Bile Salt Hydrolase Activity in the Food-Derived Strain Levilactobacillus brevis M3R3: Genomic and Functional Characterization.Probiotics and antimicrobial proteins · 2026Article
- Direct-fed Microbials (DFM) and Poultry Genomics: A Synergistic Approach to Sustainable Antibiotic Free Farming.Probiotics and antimicrobial proteins · 2026Review
- Interaction BetweenCurrent pharmaceutical biotechnology · 2026Review
- Unravelling the key factors for the dominance of Leuconostoc starters during kimchi fermentation.NPJ science of food · 2025Article
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- Regulatory mechanisms and applications ofFrontiers in microbiology · 2024Review
- Proteomic analysis to unravel the biochemical mechanisms triggered by Bacillus toyonensis SFC 500-1E under chromium(VI) and phenol stress.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Non-inhibitory levels of oxygen during cultivation increase freeze-drying stress tolerance inFrontiers in microbiology · 2023Article
- Genome Sequence and Assessment of Safety and Potential Probiotic Traits ofMicroorganisms · 2022Article
- Strategy of Developing Oral Vaccine Candidates Against Co-infection of Porcine Diarrhea Viruses Based on aFrontiers in microbiology · 2022Article
- Significance of Individual Domains of ClpL: A Novel Chaperone fromBiochemistry · 2020Article
- Adaptation of Lactobacillus plantarum to Ampicillin Involves Mechanisms That Maintain Protein Homeostasis.mSystems · 2020Article
- The Ability of Riboflavin-OverproducingFrontiers in microbiology · 2020Article
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- Challenges and Pitfalls in the Engineering of Human Interleukin 22 (hIL-22) SecretingFrontiers in bioengineering and biotechnology · 2020Article
- Comparative Analysis ofToxins · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To be able to function as a probiotic, bacteria have to survive the passage through the gastrointestinal tract. We have examined survival and gene expression of Lactobacillus reuteri ATCC 55730 after a sudden shift in environmental acidity to a pH close to the conditions in the human stomach. More than 80% of the L. reuteri cells survived at pH 2.7 for 1 h. A genomewide expression analysis experiment using microarrays displayed 72 differentially expressed genes at this pH. The early response to severe acid shock in L. reuteri differed from long-term acid adaptation to milder acid stress studied in other lactic acid bacteria. The genes induced included the following: clpL, genes putatively involved in alterations of the cell membrane and the cell wall; genes encoding transcriptional regulators; phage genes; and genes of unknown function. Two genes, clpL, encoding an ATPase with chaperone activity, and lr1516, encoding a putative esterase, were selected for mutation analyses. The mutants were significantly more sensitive to acid than the wild type was. Thus, these genes could contribute to the survival of L. reuteri in the gastrointestinal tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.