ArticleBiophysical journal2007
Two-step mechanism of homogeneous nucleation of sickle cell hemoglobin polymers.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 113 citations in OpenAlex.
- Navigating Classical and Nonclassical Realms of Nucleation and Crystallization.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Classical and Nonclassical Nucleation Mechanisms of Biomolecules.Advances in biochemical engineering/biotechnology · 2026Review
- Accompanying Hemoglobin Polymerization in Red Blood Cells in Patients with Sickle Cell Disease Using Fluorescence Lifetime Imaging.International journal of molecular sciences · 2024Article
- Protein nanocondensates: the next frontier.Biophysical reviews · 2023Review
- Fluorescence Lifetime Measurement of Prefibrillar Sickle Hemoglobin Oligomers as a Platform for Drug Discovery in Sickle Cell Disease.Biomacromolecules · 2022Article
- Protein conformation and biomolecular condensates.Current research in structural biology · 2022Review
- A multi-step nucleation process determines the kinetics of prion-like domain phase separation.Nature communications · 2021Article
- Mesoscopic protein-rich clusters host the nucleation of mutant p53 amyloid fibrils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Sequestration within biomolecular condensates inhibits Aβ-42 amyloid formation.Chemical science · 2021Article
- Dynamics of oligomer populations formed during the aggregation of Alzheimer's Aβ42 peptide.Nature chemistry · 2020Article
- Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease.ACS sensors · 2019Article
- Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions.The journal of physical chemistry. B · 2019Article
- Molecular insights into the irreversible mechanical behavior of sickle hemoglobin.Journal of biomolecular structure & dynamics · 2019Article
- Anomalous Dense Liquid Condensates Host the Nucleation of Tumor Suppressor p53 Fibrils.iScience · 2019Article
- Hemoglobin to Hematocrit Ratio: The Strongest Predictor of Femoral Head Osteonecrosis in Children With Sickle Cell Disease.Journal of pediatric orthopedics · 2016Article
- Lack of Dependence of the Sizes of the Mesoscopic Protein Clusters on Electrostatics.Biophysical journal · 2015Article
- Photophysical characterization of sickle cell disease hemoglobin by multi-photon microscopy.Biomedical optics express · 2015Article
- Do protein crystals nucleate within dense liquid clusters?Acta crystallographica. Section F, Structural biology communications · 2015Article
- Crucial role of nonspecific interactions in amyloid nucleation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Nucleation precursors in protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Sickle cell anemia is a debilitating genetic disease that affects hundreds of thousands of babies born each year worldwide. Its primary pathogenic event is the polymerization of a mutant, sickle cell, hemoglobin (HbS); and this is one of a line of diseases (Alzheimer's, Huntington's, prion, etc.) in which nucleation initiates pathophysiology. We show that the homogeneous nucleation of HbS polymers follows a two-step mechanism with metastable dense liquid clusters serving as precursor to the ordered nuclei of the HbS polymer. The evidence comes from data on the rates of fiber nucleation and growth and nucleation delay times, the interaction of fibers with polarized light, and mesoscopic metastable HbS clusters in solution. The presence of a precursor in the HbS nucleation mechanism potentially allows low-concentration solution components to strongly affect the nucleation kinetics. The variations of these concentrations in patients might account for the high variability of the disease in genetically identical patients. In addition, these components can potentially be utilized for control of HbS polymerization and treatment of the disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.