ArticleBiophysical journal2007
Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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Who cites it
44 citing papers in PubMed, 126 citations in OpenAlex.
- Increased Disarray of Extracellular Matrix Collagen-I Fiber Network and Compromised Biomechanics in Aortae From Marfan-Syndrome Mice Assessed Through Combined Opto-Biomechatronics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.Communications biology · 2025Article
- Handheld multiphoton and pinhole-free reflectance confocal microscopy enables noninvasive, real-time cross-sectional imaging in skin.Scientific reports · 2024Article
- Exogenous Nucleotides Improve the Skin Aging of SAMP8 Mice by Modulating Autophagy through MAPKs and AMPK Pathways.Nutrients · 2024Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Imaging guidance for cholesteatoma surgery using tissue autofluorescence.Journal of biomedical optics · 2023Article
- Deep-skin multiphoton microscopy of lymphatic vessels excited at the 1700-nm window in vivo.Biomedical optics express · 2021Article
- Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model ofFrontiers in medicine · 2021Article
- Intravital Metabolic Autofluorescence Imaging Captures Macrophage Heterogeneity Across Normal and Cancerous Tissue.Frontiers in bioengineering and biotechnology · 2021Article
- Label-free spectroscopic tissue characterization using fluorescence excitation-scanning spectral imaging.Journal of biophotonics · 2020Article
- Simultaneous label-free autofluorescence and multi-harmonic imaging revealsBiomedical optics express · 2019Article
- Custom Multiphoton/Raman Microscopy Setup for Imaging and Characterization of Biological Samples.Methods and protocols · 2019Article
- Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice.Lasers in medical science · 2018Article
- Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro-spectroscopy.Lasers in surgery and medicine · 2017Article
- Development of thin skin mimicking bilayer solid tissue phantoms for optical spectroscopic studies.Biomedical optics express · 2017Article
- In vivo imaging system for explants analysis-A new approach for assessment of cell transplantation effects in large animal models.PloS one · 2017Article
- Microstructure and mechanics of human resistance arteries.American journal of physiology. Heart and circulatory physiology · 2016Article
- Non-invasive Assessments of Adipose Tissue Metabolism In Vitro.Annals of biomedical engineering · 2016Review
- Influence of Autofluorescence Derived From Living Body on In Vivo Fluorescence Imaging Using Quantum Dots.Cell medicine · 2015Article
- Granzyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation.Aging cell · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The deep tissue penetration and submicron spatial resolution of multiphoton microscopy and the high detection efficiency and nanometer spectral resolution of a spectrograph were utilized to record spectral images of the intrinsic emission of mouse skin tissues. Autofluorescence from both cellular and extracellular structures, second-harmonic signal from collagen, and a narrowband emission related to Raman scattering of collagen were detected. Visualization of the spectral images by wavelength-to-RGB color image conversion allowed us to identify and discriminate tissue structures such as epidermal keratinocytes, lipid-rich corneocytes, intercellular structures, hair follicles, collagen, elastin, and dermal cells. Our results also showed morphological and spectral differences between excised tissue section, thick excised tissue, and in vivo tissue samples of mouse skin. Results on collagen excitation at different wavelengths suggested that the origin of the narrowband emission was collagen Raman peaks. Moreover, the oscillating spectral dependency of the collagen second-harmonic intensity was experimentally studied. Overall, spectral imaging provided a wealth of information not easily obtainable with present conventional multiphoton imaging systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.