ArticlePlant physiology2007
Gene targeting by homologous recombination as a biotechnological tool for rice functional genomics.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice.Plants (Basel, Switzerland) · 2022Article
- Targeted DNA insertion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Can genetic engineering-based methods for gene function identification be eclipsed by genome editing in plants? A comparison of methodologies.Molecular genetics and genomics : MGG · 2021Review
- Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.Plant biotechnology (Tokyo, Japan) · 2021Article
- Precision genome editing in plants via gene targeting and subsequent break-induced single-strand annealing.Plant biotechnology journal · 2021Article
- Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting.Planta · 2021Article
- Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research.Biodesign research · 2020Review
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice.Data in brief · 2018Article
- Transcriptional regulatory mechanism of alcohol dehydrogenase 1-deficient mutant of rice for cell survival under complete submergence.Rice (New York, N.Y.) · 2016Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Biallelic Gene Targeting in Rice.Plant physiology · 2016Article
- Precise Genome Modification via Sequence-Specific Nucleases-Mediated Gene Targeting for Crop Improvement.Frontiers in plant science · 2016Review
- Transcription Activator-Like Effector Nucleases (TALEN)-Mediated Targeted DNA Insertion in Potato Plants.Frontiers in plant science · 2016Article
- Gene Targeting Without DSB Induction Is Inefficient in Barley.Frontiers in plant science · 2016Article
- Article
- Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA.Plant physiology · 2015Article
- Cas9-Guide RNA Directed Genome Editing in Soybean.Plant physiology · 2015Article
- A Universal Positive-Negative Selection System for Gene Targeting in Plants Combining an Antibiotic Resistance Gene and Its Antisense RNA.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The modification of an endogenous gene into a designed sequence by homologous recombination, termed gene targeting (GT), has broad implications for basic and applied research. Rice (Oryza sativa), with a sequenced genome of 389 Mb, is one of the most important crops and a model plant for cereals, and the single-copy gene Waxy on chromosome 6 has been modified with a frequency of 1% per surviving callus by GT using a strong positive-negative selection. Because the strategy is independent of gene-specific selection or screening, it is in principle applicable to any gene. However, a gene in the multigene family or a gene carrying repetitive sequences may preclude efficient homologous recombination-promoted GT due to the occurrence of ectopic recombination. Here, we describe an improved GT procedure whereby we obtained nine independent transformed calli having the alcohol dehydrogenase2 (Adh2) gene modified with a frequency of approximately 2% per surviving callus and subsequently isolated eight fertile transgenic plants without the concomitant occurrence of undesirable ectopic events, even though the rice genome carries four Adh genes, including a newly characterized Adh3 gene, and a copy of highly repetitive retroelements is present adjacent to the Adh2 gene. The results indicate that GT using a strong positive-negative selection can be widely applicable to functional genomics in rice and presumably in other higher plants.
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