ArticleVirology2007
Characterization of a baculovirus lacking the DBP (DNA-binding protein) gene.
Article in Virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Baculoviruses exploit the mitotic kinase CDK1 to disrupt the nuclear lamina.PLoS pathogens · 2026Article
- A novel method for generating baculovirus bacmids using EGFP-mediated purification and linearization.Biotechnology letters · 2025Article
- Systematic Analysis of 42 Autographa Californica Multiple Nucleopolyhedrovirus Genes Identifies An Additional Six Genes Involved in the Production of Infectious Budded Virus.Virologica Sinica · 2021Article
- Genome Analysis of a Novel Clade II.bViruses · 2019Article
- Nucleocapsid Assembly of Baculoviruses.Viruses · 2019Review
- Baculovirus infection induces disruption of the nuclear lamina.Scientific reports · 2017Article
- Autographa californica multiple nucleopolyhedrovirus ORF11 is essential for budded-virus production and occlusion-derived-virus envelopment.Journal of virology · 2015Article
- The genome of a baculovirus isolated from Hemileuca sp. encodes a serpin ortholog.Virus genes · 2013Article
- Distribution and phosphorylation of the basic protein P6.9 of Autographa californica nucleopolyhedrovirus.Journal of virology · 2012Article
- Production of baculovirus defective interfering particles during serial passage is delayed by removing transposon target sites in fp25k.The Journal of general virology · 2012Article
- Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives.ISRN microbiology · 2012Article
- Article
- Baculovirus infection induces a DNA damage response that is required for efficient viral replication.Journal of virology · 2011Article
- Complete sequence, analysis and organization of the Orgyia leucostigma nucleopolyhedrovirus genome.Viruses · 2011Article
- Effect of ac68 knockout and lef3 leading sequence disruption on viral propagation.Current microbiology · 2011Article
- Sf29 gene of Spodoptera frugiperda multiple nucleopolyhedrovirus is a viral factor that determines the number of virions in occlusion bodies.Journal of virology · 2008Article
- Characterization of AcMNPV with a deletion of ac68 gene.Virus genes · 2008Article
- Isolation and characterization of the DNA-binding protein (DBP) of the Autographa californica multiple nucleopolyhedrovirus.Virology · 2008Article
- Characterization of baculovirus constructs lacking either the Ac 101, Ac 142, or the Ac 144 open reading frame.Virology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) encodes two proteins that possess properties typical of single-stranded DNA-binding proteins (SSBs), late expression factor-3 (LEF-3), and a protein referred to as DNA-binding protein (DBP). Whereas LEF-3 is a multi-functional protein essential for viral DNA replication, transporting helicase into the nucleus, and forms a stable complex with the baculovirus alkaline nuclease, the role for DBP in baculovirus replication remains unclear. Therefore, to better understand the functional role of DBP in viral replication, a DBP knockout virus was generated from an AcMNPV bacmid and analyzed. The results of a growth curve analysis indicated that the dbp knockout construct was unable to produce budded virus indicating that dbp is essential. The lack of DBP does not cause a general shutdown of the expression of viral genes, as was revealed by accumulation of early (LEF-3), late (VP39), and very late (P10) proteins in cells transfected with the dbp knockout construct. To investigate the role of DBP in DNA replication, a real-time PCR-based assay was employed and showed that, although viral DNA synthesis occurred in cells transfected with the dbp knockout, the levels were less than that of the control virus suggesting that DBP is required for normal levels of DNA synthesis or for stability of nascent viral DNA. In addition, analysis of the viral DNA replicated by the dbp knockout by using field inversion gel electrophoresis failed to detect the presence of genome-length DNA. Furthermore, analysis of DBP from infected cells indicated that similar to LEF-3, DBP was tightly bound to viral chromatin. Assessment of the cellular localization of DBP relative to replicated viral DNA by immunoelectron microscopy indicated that, at 24 h post-infection, DBP co-localized with nascent DNA at distinct electron-dense regions within the nucleus. Finally, immunoelectron microscopic analysis of cells transfected with the dbp knockout revealed that DBP is required for the production of normal-appearing nucleocapsids and for the generation of the virogenic stroma.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.