ArticleJournal of applied microbiology2007
In vivo gene delivery and expression by bacteriophage lambda vectors.
Article in Journal of applied microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- Virus-like particles based on plant viruses and bacteriophages: emerging strategies for the delivery of nucleic acid therapeutics.Chemical science · 2026Review
- Bacteriophages as Targeted Therapeutic Vehicles: Challenges and Opportunities.Bioengineering (Basel, Switzerland) · 2025Review
- Phage-based delivery systems: engineering, applications, and challenges in nanomedicines.Journal of nanobiotechnology · 2024Review
- Genetic Engineering and Biosynthesis Technology: Keys to Unlocking the Chains of Phage Therapy.Viruses · 2023Review
- Review
- Bacteriophage-Mediated Cancer Gene Therapy.International journal of molecular sciences · 2022Review
- Phage engineering and the evolutionary arms race.Current opinion in biotechnology · 2021Review
- Engineered Bacteriophage T7 as a Potent Anticancer AgentFrontiers in microbiology · 2020Article
- Enhancement of Photodynamic Cancer Therapy by Physical and Chemical Factors.Angewandte Chemie (International ed. in English) · 2019Review
- Lambda phage nanoparticles displaying HER2-derived E75 peptide induce effective E75-CD8Immunologic research · 2018Article
- Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos.Advanced drug delivery reviews · 2016Review
- Bacteriophage lambda: Early pioneer and still relevant.Virology · 2015Review
- Bacteriophages and medical oncology: targeted gene therapy of cancer.Medical oncology (Northwood, London, England) · 2014Review
- Role of bacteriophages in STEC infections: new implications for the design of prophylactic and treatment approaches.F1000Research · 2014Article
- Simultaneous display of two large proteins on the head and tail of bacteriophage lambda.BMC biotechnology · 2013Article
- Communication: Origin of the contributions to DNA structure in phages.The Journal of chemical physics · 2013Article
- Article
- λ Phage nanobioparticle expressing apoptin efficiently suppress human breast carcinoma tumor growth in vivo.PloS one · 2013Article
- Architectonics of phage-liposome nanowebs as optimized photosensitizer vehicles for photodynamic cancer therapy.Molecular cancer therapeutics · 2010Article
- Plasmid transduction using bacteriophage Phi(adh) for expression of CC chemokines by Lactobacillus gasseri ADH.Applied and environmental microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
aimsBacteriophage vectors have potential as gene transfer and vaccine delivery vectors because of their low cost, safety and physical stability. However, little is known concerning phage-mediated gene transfer in mammalian hosts. We therefore performed experiments to examine phage-mediated gene transfer in vivo. METHODS AND
resultsMice were inoculated with recombinant lambda phage containing a mammalian expression cassette encoding firefly luciferase (luc). Efficient, dose-dependent in vivo luc expression was detected, which peaked within 24 h of delivery and declined to undetectable levels within a week. Display of an integrin-binding peptide increased cellular internalization of phage in vitro and enhanced phage-mediated gene transfer in vivo. Finally, in vivo depletion of phagocytic cells using clodronate liposomes had only a minor effect on the efficiency of phage-mediated gene transfer.
conclusionsUnmodified lambda phage particles are capable of transducing mammalian cells in vivo, and may be taken up -- at least in part -- by nonphagocytic mechanisms. Surface modifications that enhance phage uptake result in more efficient in vivo gene transfer. SIGNIFICANCE AND IMPACT OF THE STUDY: These experiments shed light on the mechanisms involved in phage-mediated gene transfer in vivo, and suggest new approaches that may enhance the efficiency of this process.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.