ArticleMolecular genetics and genomics : MGG2007
The role of histone ubiquitylation and deubiquitylation in gene expression as determined by the analysis of an HTB1(K123R) Saccharomyces cerevisiae strain.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Emerging Insights into the Roles of the Paf1 Complex in Gene Regulation.Trends in biochemical sciences · 2017Review
- The Histone Modification Domain of Paf1 Complex Subunit Rtf1 Directly Stimulates H2B Ubiquitylation through an Interaction with Rad6.Molecular cell · 2016Article
- Histone modifications as regulators of life and death inMicrobial cell (Graz, Austria) · 2015Review
- Functional analysis of Bre1p, an E3 ligase for histone H2B ubiquitylation, in regulation of RNA polymerase II association with active genes and transcription in vivo.The Journal of biological chemistry · 2013Article
- DNA replication origin function is promoted by H3K4 di-methylation in Saccharomyces cerevisiae.Genetics · 2012Article
- Article
- Cdc73 subunit of Paf1 complex contains C-terminal Ras-like domain that promotes association of Paf1 complex with chromatin.The Journal of biological chemistry · 2012Article
- Paf1 restricts Gcn4 occupancy and antisense transcription at the ARG1 promoter.Molecular and cellular biology · 2012Article
- Histone H2B ubiquitylation and H3 lysine 4 methylation prevent ectopic silencing of euchromatic loci important for the cellular response to heat.Molecular biology of the cell · 2011Article
- Detection and characterization of ubiquitylated H2B in mammalian cells.Methods (San Diego, Calif.) · 2011Article
- Article
- The Paf1 complex represses ARG1 transcription in Saccharomyces cerevisiae by promoting histone modifications.Eukaryotic cell · 2011Article
- The Roles of the Paf1 Complex and Associated Histone Modifications in Regulating Gene Expression.Genetics research international · 2011Article
- Mutational analysis of the C-terminal FATC domain of Saccharomyces cerevisiae Tra1.Current genetics · 2010Article
- Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation.Epigenetics · 2010Review
- Novel trans-tail regulation of H2B ubiquitylation and H3K4 methylation by the N terminus of histone H2A.Molecular and cellular biology · 2010Article
- Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin.PLoS genetics · 2009Article
- The cap binding complex influences H2B ubiquitination by facilitating splicing of the SUS1 pre-mRNA.RNA (New York, N.Y.) · 2009Article
- The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression.Genes & development · 2008Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Saccharomyces cerevisiae histone H2B is ubiquitylated at lysine 123 in a process requiring the E2-ubiquitin conjugase, Rad6. We have analyzed gene expression in a strain containing a variant of histone H2B with lysine 123 converted to arginine to address the mechanisms by which ubiquitylation and deubiquitylation of histone H2B affect gene expression. The SAGA complex component, Ubp8, is one of two proteases that remove the ubiquitin moiety at lysine 123. We show that changes in gene expression observed upon deletion of ubp8 are suppressed by htb1 ( K123R ), which provides genetic evidence that Ubp8 alters gene expression through deubiquitylation of histone H2B. Microarray analyses of the htb1 ( K123R ) strain show that loss of histone ubiquitylation results in a twofold or greater change in expression of approximately 1.5% of the protein coding genes with approximately 75% of these increasing. For genes in which ubiquitylation represses expression, ubiquitylation principally acts through its effects on histone methylation. In contrast, decreased expression of the CWP1 gene was not paralleled by deletions of methyltransferase components and is thus likely independent of methylation. Finally, by comparing gene expression changes in the htb1 ( K123R ) strain with those in a strain deleted for rad6, we conclude that lysine 123 affects transcription primarily because of it being a site of ubiquitylation.
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