ArticleThe EMBO journal2007
Translation of nonSTOP mRNA is repressed post-initiation in mammalian cells.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A meta-analysis of single base-pair substitutions in translational termination codons ('nonstop' mutations) that cause human inherited disease.Human genomics · 2011Pooled it
- Nonstop mutations cause loss of renal tumor suppressor proteinsScience advances · 2025Article
- Chemical inhibition of the integrated stress response impairs the ubiquitin-proteasome system.Communications biology · 2024Article
- Remodeling of the ribosomal quality control and integrated stress response by viral ubiquitin deconjugases.Nature communications · 2023Article
- GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control.Molecular cell · 2020Article
- Quality controls induced by aberrant translation.Nucleic acids research · 2020Review
- Magnesium-sensitive upstream ORF controls PRL phosphatase expression to mediate energy metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Interplay between the RNA Decay and Translation Machinery in Eukaryotes.Cold Spring Harbor perspectives in biology · 2018Review
- Lost in Translation: Ribosome-Associated mRNA and Protein Quality Controls.Frontiers in genetics · 2018Review
- Ubiquitination of stalled ribosome triggers ribosome-associated quality control.Nature communications · 2017Article
- Noncanonical Alternative Polyadenylation Contributes to Gene Regulation in Response to Hypoxia.The Plant cell · 2017Article
- The Role of MicroRNAs in Myocardial Infarction: From Molecular Mechanism to Clinical Application.International journal of molecular sciences · 2017Review
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- Article
- Splicing of Friend Murine Leukemia Virus env-mRNA Enhances Its Ability to Form Polysomes.Frontiers in microbiology · 2016Article
- The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors.Scientific reports · 2015Article
- Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae.Molecular and cellular biology · 2014Article
- Protecting the proteome: Eukaryotic cotranslational quality control pathways.The Journal of cell biology · 2014Review
- The Hbs1-Dom34 protein complex functions in non-stop mRNA decay in mammalian cells.The Journal of biological chemistry · 2013Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the fate of aberrant mRNAs lacking in-frame termination codons (called nonSTOP mRNA) in mammalian cells. We found that translation of nonSTOP mRNA was considerably repressed although a corresponding reduction of mRNA was not observed. The repression appears to be post-initiation since (i) repressed nonSTOP mRNAs were associated with polysomes, (ii) translation of IRES-initiated and uncapped nonSTOP mRNA were repressed, and (iii) protein production from nonSTOP mRNA associating with polysomes was significantly reduced when used to program an in vitro run-off translation assay. NonSTOP mRNAs distributed into lighter polysome fractions compared to control mRNAs encoding a stop codon, and a significant amount of heterogeneous polypeptides were produced during in vitro translation of nonSTOP RNAs, suggesting premature termination of ribosomes translating nonSTOP mRNA. Moreover, a run-off translation assay using hippuristanol and RNAse protection assays suggested the presence of a ribosome stalled at the 3' end of nonSTOP mRNAs. Taken together, these data indicate that ribosome stalling at the 3' end of nonSTOP mRNAs can block translation by preventing upstream translation events.
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