ArticleThe EMBO journal2007
Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 84 citations in OpenAlex.
- Ubiquitin and its relatives as wizards of the endolysosomal system.Journal of cell science · 2023Article
- Bro1 binds the Vps20 subunit of ESCRT-III and promotes ESCRT-III regulation by Doa4.Traffic (Copenhagen, Denmark) · 2022Article
- Vacuolar H+-ATPase dysfunction rescues intralumenal vesicle cargo sorting in yeast lacking PI(3,5)P2 or Doa4.Journal of cell science · 2021Article
- HIV-1 Gag release from yeast reveals ESCRT interaction with the Gag N-terminal protein region.The Journal of biological chemistry · 2020Article
- The structure and function of deubiquitinases: lessons from budding yeast.Open biology · 2020Review
- The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion.Molecular and cellular neurosciences · 2020Article
- TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy.The Journal of cell biology · 2020Article
- Ubiquitin-specific protease 8 (USP8/UBPy): a prototypic multidomain deubiquitinating enzyme with pleiotropic functions.Biochemical Society transactions · 2019Review
- Dissecting the role of His domain protein tyrosine phosphatase/PTPN23 and ESCRTs in sorting activated epidermal growth factor receptor to the multivesicular body.Biochemical Society transactions · 2018Review
- Ubiquitin turnover and endocytic trafficking in yeast are regulated by Ser57 phosphorylation of ubiquitin.eLife · 2017Article
- ESCRT-mediated vesicle concatenation in plant endosomes.The Journal of cell biology · 2017Article
- Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase.Molecular biology of the cell · 2017Article
- ESCRT-Dependent Cell Death in a Caenorhabditis elegans Model of the Lysosomal Storage Disorder Mucolipidosis Type IV.Genetics · 2016Article
- Electron microscopy for ultrastructural analysis and protein localization inMicrobial cell (Graz, Austria) · 2015Review
- Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Conserved Mode of Interaction between Yeast Bro1 Family V Domains and YP(X)nL Motif-Containing Target Proteins.Eukaryotic cell · 2015Article
- Review
- The ESCRT-III adaptor protein Bro1 controls functions of regulator for free ubiquitin chains 1 (Rfu1) in ubiquitin homeostasis.The Journal of biological chemistry · 2014Article
- Binding to any ESCRT can mediate ubiquitin-independent cargo sorting.Traffic (Copenhagen, Denmark) · 2014Article
- UBE4B protein couples ubiquitination and sorting machineries to enable epidermal growth factor receptor (EGFR) degradation.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Doa4 is a ubiquitin-specific protease in Saccharomyces cerevisiae that deubiquitinates integral membrane proteins sorted into the lumenal vesicles of late-endosomal multivesicular bodies (MVBs). We show that the non-catalytic N terminus of Doa4 mediates its recruitment to endosomes through its association with Bro1, which is one of several highly conserved class E Vps proteins that comprise the core MVB sorting machinery. In turn, Bro1 directly stimulates deubiquitination by interacting with a YPxL motif in the catalytic domain of Doa4. Mutations in either Doa4 or Bro1 that disrupt catalytic activation of Doa4 impair deubiquitination and sorting of MVB cargo proteins and lead to the formation of lumenal MVB vesicles that are predominantly small compared with the vesicles seen in wild-type cells. Thus, by recruiting Doa4 to late endosomes and stimulating its catalytic activity, Bro1 fulfills a novel dual role in coordinating deubiquitination in the MVB pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.