ArticleMolecular genetics and genomics : MGG2007
In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.
Article in Molecular genetics and genomics : MGG, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L.Nucleic acids research · 2026Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Component analysis of nucleolar protein compartments using Xenopus laevis oocytes.Development, growth & differentiation · 2022Article
- The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40S ribosome assembly.RNA (New York, N.Y.) · 2022Article
- Computational Characterizing Necroptosis Reveals Implications for Immune Infiltration and Immunotherapy of Hepatocellular Carcinoma.Frontiers in oncology · 2022Article
- Article
- Upregulation of GTPBP4 Promotes the Proliferation of Liver Cancer Cells.Journal of oncology · 2021Article
- Insights into remodeling events during eukaryotic large ribosomal subunit assembly provided by high resolution cryo-EM structures.RNA biology · 2017Review
- The small GTPase, nucleolar GTP-binding protein 1 (NOG1), has a novel role in plant innate immunity.Scientific reports · 2017Article
- Principles of 60S ribosomal subunit assembly emerging from recent studies in yeast.The Biochemical journal · 2017Review
- Suppression of NGB and NAB/ERabp1 in tomato modifies root responses to potato cyst nematode infestation.Molecular plant pathology · 2015Article
- Nucleolar GTPase NOG-1 regulates development, fat storage, and longevity through insulin/IGF signaling in C. elegans.Molecules and cells · 2014Article
- Article
- A protein domain-centric approach for the comparative analysis of human and yeast phenotypically relevant mutations.BMC genomics · 2013Article
- Saccharomyces cerevisiae ribosomal protein L26 is not essential for ribosome assembly and function.Molecular and cellular biology · 2012Article
- Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA.Molecular and cellular biology · 2010Article
- A genome-scale protein interaction profile of Drosophila p53 uncovers additional nodes of the human p53 network.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1.Eukaryotic cell · 2009Article
- A mutant plasma membrane protein is stabilized upon loss of Yvh1, a novel ribosome assembly factor.Genetics · 2009Article
- E. coli HflX interacts with 50S ribosomal subunits in presence of nucleotides.Biochemical and biophysical research communications · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae Nog1 GTPase is critical for assembly of the large ribosomal subunit. Mutations in conserved residues in the GTP-binding pocket cause defects in cell growth and 60S ribosome assembly but mutant proteins retain their ability to associate with the pre-60S. Association of Nog1 with the pre-60S is independent of guanine nucleotide added to cell extracts. Thus, it appears that nucleotide occupancy does not substantially affect Nog1 association with pre-60S particles. Somewhat surprisingly, neither of the conserved threonines in the G2 motif of the GTPase domain is essential for Nog1 function. Neither the steady-state rRNA levels nor the protein composition (as determined by isobaric labeling and identification by mass spectrometry of peptides) of the pre-60S particles in the nog1P176V mutant are grossly perturbed, although levels of four proteins (Nog1, Nop2, Nop15, and Tif6) are modestly reduced in pre-60S particles isolated from the mutant. Deletion analysis revealed that the C-terminal 168 amino acids are not required for function; however, the N-terminal 126 amino acids are required. Optimal association with pre-60S particles requires sequences between amino acids 347-456. Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.