ArticleGene therapy2007
Strategies for CNS-directed gene delivery: in vivo gene transfer to the brain using SV40-derived vectors.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Transfection of primary brain capillary endothelial cells for protein synthesis and secretion of recombinant erythropoietin: a strategy to enable protein delivery to the brain.Cellular and molecular life sciences : CMLS · 2017Article
- Role of Oxidative Stress in HIV-1-Associated Neurocognitive Disorder and Protection by Gene Delivery of Antioxidant Enzymes.Antioxidants (Basel, Switzerland) · 2014Review
- Oxidative Stress Is Associated with Neuroinflammation in Animal Models of HIV-1 Tat Neurotoxicity.Antioxidants (Basel, Switzerland) · 2014Article
- The glial response to intracerebrally delivered therapies for neurodegenerative disorders: is this a critical issue?Frontiers in pharmacology · 2014Review
- Current approaches to enhance CNS delivery of drugs across the brain barriers.International journal of nanomedicine · 2014Review
- Article
- Gene delivery of antioxidant enzymes inhibits human immunodeficiency virus type 1 gp120-induced expression of caspases.Neuroscience · 2012Article
- Intracisternal rSV40 administration provides effective pan-CNS transgene expression.Gene therapy · 2012Article
- Blood-brain barrier abnormalities caused by HIV-1 gp120: mechanistic and therapeutic implications.TheScientificWorldJournal · 2012Review
- Gene transfer to the rhesus monkey brain using SV40-derived vectors is durable and safe.Gene therapy · 2011Article
- Role of CCR5 and its ligands in the control of vascular inflammation and leukocyte recruitment required for acute excitotoxic seizure induction and neural damage.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Thinking about HIV: the intersection of virus, neuroinflammation and cognitive dysfunction.Immunologic research · 2010Review
- Gene transfer to the cerebellum.Cerebellum (London, England) · 2010Article
- Efficient CNS gene delivery by intravenous injection.Nature methods · 2010Article
- HIV-1 gp120-induced injury to the blood-brain barrier: role of metalloproteinases 2 and 9 and relationship to oxidative stress.Journal of neuropathology and experimental neurology · 2010Article
- Immunohistochemical detection of transgene expression in the brain using small epitope tags.BMC biotechnology · 2010Article
- Persistent expression of FLAG-tagged micro dystrophin in nonhuman primates following intramuscular and vascular delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- A rat model of human immunodeficiency virus 1 encephalopathy using envelope glycoprotein gp120 expression delivered by SV40 vectors.Journal of neuropathology and experimental neurology · 2009Article
- Methods, potentials, and limitations of gene delivery to regenerate central nervous system cells.Biologics : targets & therapy · 2009Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Gene transfer to the central nervous system (CNS) has been approached using various vectors. Recombinant SV40-derived vectors (rSV40s) transduce neurons and microglia effectively in vitro, so we tested rSV40s gene transfer to the CNS in vivo, and characterized the distribution, duration and cell types transduced. We used rSV40s carrying Human Immunodeficiency Virus Type 1 Net protein (HIV-1 Nef) with a C-terminal FLAG epitope tag as a marker, and another with Cu/Zn superoxide dismutase (SOD1). Rats were given vectors stereotaxically, either intraparenchymally into the caudate-putamen (CP) or into the lateral ventricle (LV). FLAG expression was studied for 3 months by immunostaining serial brain sections. After intraparenchymal administration, numerous transgene-expressing cells were seen, many as far as 4 mm from the injection site. Transgene expression remained strong throughout the 3-month study period. Coimmunostaining for lineage markers showed that neurons and, more rarely, microglial cells were tranduced, except astrocytes and oligodendroglia. After injection into the LV, high levels of transgene expression were detected throughout the frontal cortex by Western analysis. Systemic mannitol-induced hyperosmolarity further augmented LV transgene delivery. SV40-derived vectors may, thus, be useful for long-term gene expression in the brain, whether locally by intraparenchymal administration or diffusely by intraventricular injection, with or without mannitol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.