ArticleJournal of clinical microbiology2007
Molecular discrimination of atypical bovine spongiform encephalopathy strains from a geographical region spanning a wide area in Europe.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
81 citing papers in PubMed, 176 citations in OpenAlex.
- Bovine Spongiform Encephalopathy: An Integrated Review of Prion Mechanisms, Neuroanatomy, and Control.Veterinary sciences · 2026Review
- Strain Traits of Intracranially Administered L-Type Bovine Spongiform Encephalopathy Prions Are not Significantly Modified During Intraspecies Transmission in Cynomolgus Monkeys.Microbiology and immunology · 2026Article
- Co-presence of classical scrapie but not classical Bovine Spongiform Encephalopathy in transmissions from Dutch sheep with atypical scrapie.The Journal of general virology · 2025Article
- Article
- Experimental transmission of ovine atypical scrapie to cattle.Veterinary research · 2023Article
- Conventional and State-of-the-Art Detection Methods of Bovine Spongiform Encephalopathy (BSE).International journal of molecular sciences · 2023Review
- Short incubation periods of atypical H-type BSE in cattle with EK211 and KK211 prion protein genotypes after intracranial inoculation.Frontiers in veterinary science · 2023Article
- The First Evaluation of Proteinase K-Resistant Prion Protein (PrPMedicina (Kaunas, Lithuania) · 2022Article
- Second passage of chronic wasting disease of mule deer to sheep by intracranial inoculation compared to classical scrapie.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- The ultrastructure of infectious L-type bovine spongiform encephalopathy prions constrains molecular models.PLoS pathogens · 2021Article
- Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein.Veterinary research · 2021Article
- Article
- Influence of Interspecies Transmission of Atypical Bovine Spongiform Encephalopathy Prions to Hamsters on Prion Characteristics.Frontiers in veterinary science · 2020Article
- Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods.Veterinary research · 2019Article
- Estimation of prion infectivity in tissues of cattle infected with atypical BSE by real time-quaking induced conversion assay.The Journal of veterinary medical science · 2019Article
- Bovine Spongiform Encephalopathy - A Review from the Perspective of Food Safety.Food safety (Tokyo, Japan) · 2019Review
- Article
- Scientific opinion on chronic wasting disease (II).EFSA journal. European Food Safety Authority · 2018Article
- Article
- Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats.PloS one · 2018Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmissible spongiform encephalopathy strains can be differentiated by their behavior in bioassays and by molecular analyses of the disease-associated prion protein (PrP) in a posttranslationally transformed conformation (PrPSc). Until recently, isolates from cases of bovine spongiform encephalopathy (BSE) appeared to be very homogeneous. However, a limited number of atypical BSE isolates have recently been identified upon analyses of the disease-associated proteinase K (PK) resistance-associated moiety of PrPSc (PrPres), suggesting the existence of at least two additional BSE PrPres variants. These are defined here as the H type and the L type, according to the higher and lower positions of the nonglycosylated PrPres band in Western blots, respectively, compared to the position of the band in classical BSE (C-type) isolates. These molecular PrPres variants, which originated from six different European countries, were investigated together. In addition to the migration properties and glycosylation profiles (glycoprofiles), the H- and L-type isolates exhibited enhanced PK sensitivities at pH 8 compared to those of the C-type isolates. Moreover, H-type BSE isolates exhibited differences in the binding of antibodies specific for N- and more C-terminal PrP regions and principally contained two aglycosylated PrPres moieties which can both be glycosylated and which is thus indicative of the existence of two PrPres populations or intermediate cleavage sites. These properties appear to be consistent within each BSE type and independent of the geographical origin, suggesting the existence of different BSE strains in cattle. The choice of three antibodies and the application of two pHs during the digestion of brain homogenates provide practical and diverse tools for the discriminative detection of these three molecular BSE types and might assist with the recognition of other variants.
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