ArticleImmunogenetics2007
Contribution of single nucleotide polymorphisms of the IL1A gene to the cleavage of precursor IL-1alpha and its transcription activity.
Article in Immunogenetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Gene variants associated with acne vulgaris presentation and severity: a systematic review and meta-analysis.BMC medical genomics · 2021Pooled it
- Prediction of the most deleterious non-synonymous SNPs in the human IL1B gene: evidence from bioinformatics analyses.BMC genomic data · 2024Article
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- Chlamydia evasion of neutrophil host defense results in NLRP3 dependent myeloid-mediated sterile inflammation through the purinergic P2X7 receptor.Nature communications · 2021Article
- Review
- Genetic Factors Underlying Sudden Infant Death Syndrome.The application of clinical genetics · 2021Review
- IL-1A gene variation in relation to cytokine levels and clinical characteristics in ankylosing spondylitis.European journal of rheumatology · 2019Article
- Association between IL1A and IL1B polymorphisms and primary open angle glaucoma in a Brazilian population.Experimental biology and medicine (Maywood, N.J.) · 2018Article
- Function and regulation of IL-1α in inflammatory diseases and cancer.Immunological reviews · 2018Review
- Interleukin 1α and the inflammatory process.Nature immunology · 2016Review
- Interleukin polymorphisms associated with overall survival, disease-free survival, and recurrence in non-small cell lung cancer patients.Molecular carcinogenesis · 2015Article
- Association of interleukin 1 family with systemic sclerosis.Inflammation · 2014Article
- Risk of ovarian cancer and the NF-κB pathway: genetic association with IL1A and TNFSF10.Cancer research · 2014Article
- The immune system in the pathogenesis of ovarian cancer.Critical reviews in immunology · 2013Review
- Gender difference in genetic association between IL1A variant and early lumbar disc degeneration: a three-year follow-up.International journal of molecular epidemiology and genetics · 2012Article
- Functional polymorphism of IL-1 alpha and its potential role in obesity in humans and mice.PloS one · 2011Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated the association of IL1A gene single nucleotide polymorphisms (SNPs) with susceptibility to systemic sclerosis (SSc) patients. In this study, we explored the effects of SNP on the transcriptional activity and processing of the precursor IL-1alpha (pre-IL-1alpha) in skin fibroblasts. Two kinds of promoter regions of the IL1A gene were prepared including C or T at -889, referred to C/IL1A and T/IL1A, and inserted into a luciferase reporter vector (pGL3). Skin fibroblasts were explanted from two SSc patients whose genomic DNA contained GG and TT genotypes at +4845 of the IL1A gene, respectively. Cell lysates were collected and reacted with various concentrations of calpain, and then the processing of pre-IL-1alpha was analyzed by Western blotting using monoclonal anti-IL-1alpha antibody. A SNP was determined by the allelic discrimination method using fluorescence-labeled Taq-Man probes. Significant differences in the luciferase activities were not detected between pGL3 (C/IL1A) and pGL3 (T/IL1A) in SSc fibroblasts. Calpain required a 100-fold higher concentration to process the pre-IL-1alpha containing Ala at the 114th amino acid than that to do containing Ser. The frequency of the GG genotype was significantly higher in SSc patients than that in healthy donors, whereas the frequency of TT genotype was significantly higher in RA patients than that in healthy donors. Our observation showed that the SNP at +4845 affected the enzymatic efficiency of the protease in cleaving pre-IL-1alpha. Pre-IL-1alpha with Ala, which was high in frequency in SSc patients, was more resistant to be cleaved by proteases in human sera than pro-IL-1alpha with Ser.
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