ArticleNucleic acids research2007
Loop II of DNA polymerase beta is important for polymerization activity and fidelity.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- A pre-catalytic non-covalent step governs DNA polymerase β fidelity.Nucleic acids research · 2019Article
- Fluorescence resonance energy transfer studies of DNA polymerase β: the critical role of fingers domain movements and a novel non-covalent step during nucleotide selection.The Journal of biological chemistry · 2014Article
- Structures of the Leishmania infantum polymerase beta.DNA repair · 2014Article
- Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase β.Biochemistry · 2013Article
- A germline polymorphism of DNA polymerase beta induces genomic instability and cellular transformation.PLoS genetics · 2012Article
- DNA polymerase family X: function, structure, and cellular roles.Biochimica et biophysica acta · 2010Review
- Loop II of DNA polymerase beta is important for discrimination during substrate binding.DNA repair · 2009Article
- The Asp285 variant of DNA polymerase beta extends mispaired primer termini via increased nucleotide binding.Biochemistry · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The accurate replication and transmission of genetic information is critical in the life of an organism. During its entire lifespan, the genetic information is constantly under attack from endogenous and exogenous sources of damage. To ensure that the content of its genetic information is faithfully preserved for synthesis and transmission, eukaryotic cells have developed a complex system of genomic quality control. Key players in this process are DNA polymerases, the enzymes responsible for synthesizing the DNA, because errors introduced into the genome by polymerase can result in mutations. We use DNA polymerase beta (pol beta) as a model system to investigate mechanisms of preserving fidelity during nucleotide incorporation. In the study described here, we characterized the role that loop II of pol beta plays in maintaining the activity and fidelity of pol beta. We report here that the absence or shortening of loop II compromises the catalytic activity of pol beta. Our data also show that loop variants of a specific length have a lower fidelity when compared to the wild-type polymerase. Taken together, our results indicate that loop II is important for the catalytic activity and fidelity of pol beta.
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Registered trials
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