ArticleProceedings of the National Academy of Sciences of the United States of America2007
Bacteriophage N4 virion RNA polymerase interaction with its promoter DNA hairpin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mini-Hairpin DNA: Extraordinarily Stable Structure and Its Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- An N4-likeJournal of bacteriology · 2026Article
- Phage-mediated control of melioidosis in Southern China: molecular insights and therapeutic potential of N4-like viruses from Hainan.BMC microbiology · 2025Article
- An N4-likebioRxiv : the preprint server for biology · 2025Article
- Isolation and characterization of a Vibrio owensii phage phi50-12.Scientific reports · 2022Article
- Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL.The EMBO journal · 2021Article
- Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases.Biomolecules · 2015Review
- Molecular determinants responsible for recognition of the single-stranded DNA regulatory sequence, χ, by RecBCD enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Molecular and physiological analysis of three Pseudomonas aeruginosa phages belonging to the "N4-like viruses".Virology · 2010Article
- Lagging-strand DNA replication origins are required for conjugal transfer of the promiscuous plasmid pMV158.Journal of bacteriology · 2009Article
- Identification of bacteriophage N4 virion RNA polymerase-nucleic acid interactions in transcription complexes.The Journal of biological chemistry · 2009Article
- Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase.Molecular cell · 2008Article
- X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- SSB as an organizer/mobilizer of genome maintenance complexes.Critical reviews in biochemistry and molecular biologyReview
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3 authors.
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Abstract
Bacteriophage N4 minivirion RNA polymerase (mini-vRNAP), the RNA polymerase (RNAP) domain of vRNAP, is a member of the T7-like RNAP family. Mini-vRNAP recognizes promoters that comprise conserved sequences and a 3-base loop-5-base pair (bp) stem DNA hairpin structure on single-stranded templates. Here, we defined the DNA structural and sequence requirements for mini-vRNAP promoter recognition. Mini-vRNAP binds a 20-nucleotide (nt) N4 P2 promoter deoxyoligonucleotide with high affinity (K(d) = 2 nM) to form a salt-resistant complex. We show that mini-vRNAP interacts specifically with the central base of the hairpin loop (-11G) and a base at the stem (-8G) and that the guanine 6-keto and 7-imino groups at both positions are essential for binding and complex salt resistance. The major determinant (-11G), which must be presented to mini-vRNAP in the context of a hairpin loop, appears to interact with mini-vRNAP Trp-129. This interaction requires template single-strandedness at positions -2 and -1. Contacts with the promoter are disrupted when the RNA product becomes 11-12 nt long. This detailed description of vRNAP interaction with its promoter hairpin provides insights into RNAP-promoter interactions and explains how the injected vRNAP, which is present in one or two copies, recognizes its promoters on a single copy of the injected genome.
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