ArticleInfection and immunity2007
Identification of transferrin-binding domains in TbpB expressed by Neisseria gonorrhoeae.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae.NPJ vaccines · 2025Article
- Review
- The genes that encode the gonococcal transferrin binding proteins, TbpB and TbpA, are differentially regulated by MisR under iron-replete and iron-depleted conditions.Molecular microbiology · 2016Article
- Surface-Exposed Lipoproteins: An Emerging Secretion Phenomenon in Gram-Negative Bacteria.Trends in microbiology · 2016Review
- Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae.Infection and immunity · 2015Article
- Analysis of the outer membrane proteome and secretome of Bacteroides fragilis reveals a multiplicity of secretion mechanisms.PloS one · 2015Article
- Conserved regions of gonococcal TbpB are critical for surface exposure and transferrin iron utilization.Infection and immunity · 2013Article
- The transferrin-iron import system from pathogenic Neisseria species.Molecular microbiology · 2012Review
- Conserved interaction between transferrin and transferrin-binding proteins from porcine pathogens.The Journal of biological chemistry · 2011Article
- TonB-Dependent Transporters Expressed by Neisseria gonorrhoeae.Frontiers in microbiology · 2011Article
- Kinetic analysis of ligand interaction with the gonococcal transferrin-iron acquisition system.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Article
- Identification and characterization of gonococcal iron transport systems as potential vaccine antigens.Future microbiology · 2008Review
- Distribution of transferrin binding protein B gene (tbpB) variants among Neisseria species.BMC microbiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The transferrin iron acquisition system of Neisseria gonorrhoeae is necessary for iron uptake from transferrin in the human host and requires the participation of two distinct proteins: TbpA and TbpB. TbpA is a TonB-dependent outer membrane transporter responsible for the transport of iron into the cell. TbpB is a lipid-modified protein, for which a precise role in receptor function has not yet been elucidated. These receptor complex proteins show promise as vaccine candidates; therefore, it is important to identify surface-exposed regions of the proteins required for wild-type functions. In this study we examined TbpB, which has been reported to be surface exposed in its entirety; however, this hypothesis has never been tested experimentally. We placed the hemagglutinin (HA) epitope into TbpB with the dual purpose of examining the surface exposure of particular epitopes as well as their impact on receptor function. Nine insertion mutants were created, placing the epitope downstream of the signal peptidase II cleavage site. We report that the HA epitope is surface accessible in all mutants, indicating that the full-length TbpB is completely surface exposed. By expressing the TbpB-HA fusion proteins in N. gonorrhoeae, we were able to examine the impact of each insertion on the function of TbpB and the transferrin acquisition process. We propose that TbpB is comprised of two transferrin-binding-competent lobes, both of which are critical for efficient iron uptake from human transferrin.
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Registered trials
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