ArticleStructure (London, England : 1993)2007
ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, sigma54.
Article in Structure (London, England : 1993), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Delineating specific regions of N- terminal domain of T3SS ATPase YsaN ofFrontiers in molecular biosciences · 2022Article
- The antiactivator FleN uses an allosteric mechanism to regulate σScience advances · 2021Article
- Article
- ATP Analogues for Structural Investigations: Case Studies of a DnaB Helicase and an ABC Transporter.Molecules (Basel, Switzerland) · 2020Review
- Mechanisms of σJournal of molecular biology · 2019Review
- Single-molecule observation of nucleotide induced conformational changes in basal SecA-ATP hydrolysis.Science advances · 2018Article
- Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.eLife · 2017Article
- Assessing heterogeneity in oligomeric AAA+ machines.Cellular and molecular life sciences : CMLS · 2017Review
- Role of the σJournal of molecular biology · 2016Article
- EF-G Activation by Phosphate Analogs.Journal of molecular biology · 2016Article
- Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.Nucleic acids research · 2014Article
- HOP2-MND1 modulates RAD51 binding to nucleotides and DNA.Nature communications · 2014Article
- Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.Nucleic acids research · 2014Article
- Breaking symmetry in multimeric ATPase motors.Cell cycle (Georgetown, Tex.) · 2014Article
- Crystallization and preliminary X-ray analysis of the ATPase domain of the σ(54)-dependent transcription activator NtrC1 from Aquifex aeolicus bound to the ATP analog ADP-BeFx.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Nucleotide-induced asymmetry within ATPase activator ring drives σ54-RNAP interaction and ATP hydrolysis.Genes & development · 2013Article
- Structural mechanism of GAF-regulated σ(54) activators from Aquifex aeolicus.Journal of molecular biology · 2013Article
- Helicase-mediated changes in RNA structure at the single-molecule level.RNA biology · 2013Review
- A common feature from different subunits of a homomeric AAA+ protein contacts three spatially distinct transcription elements.Nucleic acids research · 2012Article
- The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.Microbiology and molecular biology reviews : MMBR · 2012Review
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Authors and funding
10 authors.
Funding
Abstract
Transcription initiation by the sigma54 form of bacterial RNA polymerase requires hydrolysis of ATP by an enhancer binding protein (EBP). We present SAS-based solution structures of the ATPase domain of the EBP NtrC1 from Aquifex aeolicus in different nucleotide states. Structures of apo protein and that bound to AMPPNP or ADP-BeF(x) (ground-state mimics), ADP-AlF(x) (a transition-state mimic), or ADP (product) show substantial changes in the position of the GAFTGA loops that contact polymerase, particularly upon conversion from the apo state to the ADP-BeF(x) state, and from the ADP-AlF(x) state to the ADP state. Binding of the ATP analogs stabilizes the oligomeric form of the ATPase and its binding to sigma54, with ADP-AlF(x) having the largest effect. These data indicate that ATP binding promotes a conformational change that stabilizes complexes between EBPs and sigma54, while subsequent hydrolysis and phosphate release drive the conformational change needed to open the polymerase/promoter complex.
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