ArticleApplied and environmental microbiology2007
Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 114 citations in OpenAlex.
- Integrated Genomics and Phenotypic Analysis ofMicroorganisms · 2026Article
- Native mass spectrometry reveals DltA catalysis, DltC loading, and inhibition in the d-alanylation pathway.RSC advances · 2026Article
- Bile Salt Tolerance Determines Intestinal Colonization Efficacy ofInternational journal of molecular sciences · 2026Article
- Direct-fed Microbials (DFM) and Poultry Genomics: A Synergistic Approach to Sustainable Antibiotic Free Farming.Probiotics and antimicrobial proteins · 2026Review
- Human breast milk-derived Limosilactobacillus fermentum strains modulate hBD-2 expression against Neisseria meningitidis infection through the TLR-2/p38-MAPK/NF-κB pathway.World journal of microbiology & biotechnology · 2025Article
- Immunostimulatory activity of lipoteichoic acid with three fatty acid residues derived fromApplied and environmental microbiology · 2024Article
- Analysis of the Probiotic Potential ofMicroorganisms · 2024Article
- Genomic exploration of the fermented meat isolate Staphylococcus shinii IMDO-S216 with a focus on competitiveness-enhancing secondary metabolites.BMC genomics · 2024Article
- Molecular Mechanisms ofMicroorganisms · 2024Review
- Role of Lipoteichoic Acid from the GenusApplied and environmental microbiology · 2022Article
- Genome Sequence and Assessment of Safety and Potential Probiotic Traits ofMicroorganisms · 2022Article
- Oligosaccharide Metabolism and Lipoteichoic Acid Production inMicroorganisms · 2021Article
- iTRAQ-based quantitative analysis reveals the mechanism underlying the changes in physiological activity in a glutamate racemase mutant strain of Streptococcus mutans UA159.Molecular biology reports · 2020Article
- TheFrontiers in microbiology · 2020Article
- Lipoteichoic Acid Is Involved in the Ability of the Immunobiotic StrainFrontiers in immunology · 2020Article
- Study of theFrontiers in microbiology · 2020Article
- Impact of spray-drying on the pili of Lactobacillus rhamnosus GG.Microbial biotechnology · 2019Article
- Article
- Inhibition of Candida albicans morphogenesis by chitinase from Lactobacillus rhamnosus GG.Scientific reports · 2019Article
- A partial reconstitution implicates DltD in catalyzing lipoteichoic acid d-alanylation.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipoteichoic acid (LTA) is a macroamphiphile molecule which performs several functions in gram-positive bacteria, such as maintenance of cell wall homeostasis. D-alanylation of LTA requires the proteins encoded by the dlt operon, and this process is directly related to the charge properties of this polymer strongly contributing to its function. The insertional inactivation of dltD of the probiotic strain Lactobacillus rhamnosus GG (ATCC 53103) resulted in the complete absence of D-alanyl esters in the LTA as confirmed by nuclear magnetic resonance analysis. This was reflected in modifications of the bacterial cell surface properties. The dltD strain showed 2.4-fold-increased cell length, a low survival capacity in response to gastric juice challenge, an increased sensitivity to human beta-defensin-2, an increased rate of autolysis, an increased capacity to initiate growth in the presence of an anionic detergent, and a decreased capacity to initiate growth in the presence of cationic peptides compared to wild-type results. However, in vitro experiments revealed no major differences for adhesion to human intestinal epithelial cells, biofilm formation, and immunomodulation. These properties are considered to be important for probiotics. The role of the dlt operon in lactobacilli is discussed in view of these results.
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