ArticleJournal of molecular biology2007
Alanine scanning and Fe-BABE probing of the bacteriophage ø29 prohead RNA-connector interaction.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Constructing an active chimeric pRNA ring with a stoichiometry of six and identifying 12 domains of the pRNA ring binding to the 12-subunit channel of phi29 DNA-packaging motor.RNA (New York, N.Y.) · 2025Article
- An RNA Domain Imparts Specificity and Selectivity to a Viral DNA Packaging Motor.Journal of virology · 2015Article
- Insights into the structure and assembly of the bacteriophage 29 double-stranded DNA packaging motor.Journal of virology · 2014Article
- Discovery of a new motion mechanism of biomotors similar to the earth revolving around the sun without rotation.Virology · 2013Review
- Crystal structure of 3WJ core revealing divalent ion-promoted thermostability and assembly of the Phi29 hexameric motor pRNA.RNA (New York, N.Y.) · 2013Article
- The ATPase of the phi29 DNA packaging motor is a member of the hexameric AAA+ superfamily.Virology · 2013Article
- A three-helix junction is the interface between two functional domains of prohead RNA in 29 DNA packaging.Journal of virology · 2012Article
- Role of channel lysines and the "push through a one-way valve" mechanism of the viral DNA packaging motor.Biophysical journal · 2012Article
- Role of φ29 connector channel loops in late-stage DNA packaging.Journal of molecular biology · 2011Article
- Structure and assembly of the essential RNA ring component of a viral DNA packaging motor.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Different sequences show similar quaternary interaction stabilities in prohead viral RNA self-assembly.The Journal of biological chemistry · 2011Article
- Article
- Construction of bacteriophage phi29 DNA packaging motor and its applications in nanotechnology and therapy.Annals of biomedical engineering · 2009Review
- Novel mechanism of hexamer ring assembly in protein/RNA interactions revealed by single molecule imaging.Nucleic acids research · 2008Article
- DNA packaging motor assembly intermediate of bacteriophage phi29.Journal of molecular biology · 2008Article
- Defining molecular and domain boundaries in the bacteriophage phi29 DNA packaging motor.Structure (London, England : 1993) · 2008Article
- The effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packaging.Nanomedicine : nanotechnology, biology, and medicine · 2008Article
- Analysis of intermolecular base pair formation of prohead RNA of the phage phi29 DNA packaging motor using NMR spectroscopy.Nucleic acids research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The DNA packaging motor of the Bacillus subtilis bacteriophage ø29 prohead is comprised in part of an oligomeric ring of 174 base RNA molecules (pRNA) positioned near the N termini of subunits of the dodecameric head-tail connector. Deletion and alanine substitution mutants in the connector protein (gp10) N terminus were assembled into proheads in Escherichia coli and the particles tested for pRNA binding and DNA-gp3 packaging in vitro. The basic amino acid residues RKR at positions 3-5 of the gp10 N terminus were central to pRNA binding during assembly of an active DNA packaging motor. Conjugation of iron(S)-1-(p-bromoacetamidobenzyl) ethylenediaminetetraacetate (Fe-BABE) to residue S170C in the narrow end of the connector, near the N terminus, permitted hydroxyl radical probing of bound [(32)P]pRNA and identified two discrete sites proximal to this residue: the C-helix at the junction of the A, C and D helices, and the E helix and the CE loop/D loop of the intermolecular base pairing site.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.