ArticlePlant molecular biology2007
A barley activation tagging system.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Toward the development of Ac/Ds transposon-mediated gene tagging system for functional genomics in oat (Avena sativa L.).Functional & integrative genomics · 2022Article
- A BTB-TAZ protein is required for gene activation by Cauliflower mosaic virus 35S multimerized enhancers.Plant physiology · 2022Article
- Barley's Second Spring as A Model Organism for Chloroplast Research.Plants (Basel, Switzerland) · 2020Review
- An Efficient System forPlant physiology · 2019Article
- Development of an activation tagging system for maize.Plant direct · 2019Article
- Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance.Scientific reports · 2017Article
- Gene Overexpression Resources in Cereals for Functional Genomics and Discovery of Useful Genes.Frontiers in plant science · 2016Review
- Characterization of a potato activation-tagged mutant, nikku, and its partial revertant.Planta · 2015Article
- High-throughput generation of an activation-tagged mutant library for functional genomic analyses in tobacco.Planta · 2015Article
- Identification and use of the sugarcane bacilliform virus enhancer in transgenic maize.BMC plant biology · 2014Article
- Transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.Plant cell reports · 2014Article
- Characterization and RNA-seq analysis of underperformer, an activation-tagged potato mutant.Plant molecular biology · 2014Article
- Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection.Plant cell reports · 2013Article
- Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.BMC genomics · 2012Article
- An Ac transposon system based on maize chromosome 4S for isolating long-distance-transposed Ac tags in the maize genome.Genetica · 2010Article
- An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches.BMC plant biology · 2009Article
- Phenome analysis in plant species using loss-of-function and gain-of-function mutants.Plant & cell physiology · 2009Review
- Suppression of the barley uroporphyrinogen III synthase gene by a Ds activation tagging element generates developmental photosensitivity.The Plant cell · 2009Article
- An Ac/Ds-mediated gene trap system for functional genomics in barley.BMC genomics · 2009Article
- Activation tagging, an efficient tool for functional analysis of the rice genome.Plant molecular biology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation tagging, as the result of random genomic insertion of either promoter or enhancer sequences, can produce novel, dominant mutations by over-expression of endogenous genes. This powerful genomics tool has been used extensively in dicot species such as Arabidopsis, while rice is the only cereal for which an equivalent system exists. In this study we describe an activation tagging system in barley based upon the maize Ac/Ds transposable element system. A modified Ds element (UbiDs) containing two maize polyubiquitin promoters, transposed in families derived from multiple independent UbiDs transformants and generated new Ds insertion events at frequencies ranging from 0% to 52% per family. The majority of transposed UbiDs elements activated high levels of adjacent flanking sequence transcription. Transposon-mediated expression was detected in all barley cell and tissue types analysed suggesting that this system is applicable to all aspects of plant development and biogenesis. In addition to transcriptional activation, this system is also capable of generating insertional knockout mutants and a UbiDs inactivated allele of the granule bound starch synthase I gene (waxy) was recovered that lead to reduced amylose accumulation. The recovery and analysis of dominant over-expression phenotypes generated by this system will provide a novel approach to understanding gene function in large cereal genomes where gene redundancy may mask conventional loss-of-function mutations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.