ReviewMycorrhiza2007
Using terminal restriction fragment length polymorphism (T-RFLP) to identify mycorrhizal fungi: a methods review.
Review in Mycorrhiza, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi (Journal of fungi (Basel, Switzerland) · 2023Article
- Specific Plant Mycorrhizal Responses Are Linked to Mycorrhizal Fungal Species Interactions.Frontiers in plant science · 2022Article
- Environmental parameters and microbial community profiles as indication towards microbial activities and diversity in aquaponic system compartments.BMC microbiology · 2021Article
- Article
- Differences in the composition of arbuscular mycorrhizal fungal communities promoted by different propagule forms from a Mediterranean shrubland.Mycorrhiza · 2016Article
- Arbuscular mycorrhizal fungi in soil and roots respond differently to phosphorus inputs in an intensively managed calcareous agricultural soil.Scientific reports · 2016Article
- Article
- Diversity of Fungi on Decomposing Leaf Litter in a Sugarcane Plantation and Their Response to Tillage Practice and Bagasse Mulching: Implications for Management Effects on Litter Decomposition.Microbial ecology · 2015Article
- Site properties have a stronger influence than fire severity on ectomycorrhizal fungi and associated N-cycling bacteria in regenerating post-beetle-killed lodgepole pine forests.Folia microbiologica · 2015Article
- Article
- The interactions between plant life form and fungal traits of arbuscular mycorrhizal fungi determine the symbiotic community.Oecologia · 2014Article
- Environmental controls on fungal community composition and abundance over 3 years in native and degraded shrublands.Microbial ecology · 2014Article
- What goes in must come out: testing for biases in molecular analysis of arbuscular mycorrhizal fungal communities.PloS one · 2014Article
- Arbuscular mycorhizal fungi associated with the olive crop across the Andalusian landscape: factors driving community differentiation.PloS one · 2014Article
- Article
- Biases for detecting arbuscular mycorrhizal fungal mixture by terminal restriction fragment length polymorphism (T-RFLP).World journal of microbiology & biotechnology · 2014Article
- Fungal diversity in adult date palm (Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches.Journal of Zhejiang University. Science. B · 2013Article
- Fungal communities associated with the biodegradation of polyester polyurethane buried under compost at different temperatures.Applied and environmental microbiology · 2013Article
- Analyzing spatial patterns linked to the ecology of herbivores and their natural enemies in the soil.Frontiers in plant science · 2013Review
- Intraspecific differentiation of Colletotrichum gloeosporioides sensu lato based on in silico multilocus PCR-RFLP fingerprinting.Molecular biotechnology · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the original version) and database T-RFLP. We define database T-RFLP as the use of T-RFLP to identify individual species within samples by comparison of unknown data with a database of known T-RFLP patterns. This application of T-RFLP avoids some of the pitfalls of peak-profile T-RFLP and allows T-RFLP to be applied to polyphyletic functional groups such as ectomycorrhizal fungi. The identification of species using database T-RFLP is subject to several sources of potential error, including (1) random erroneous matches of peaks to species, (2) shared T-RFLP profiles across species, and (3) multiple T-RFLP profiles within a species. A mathematical approximation of the risk of the first type of error as a function of experimental parameters is discussed. Although potentially less accurate than some other methods such as clone libraries, the high throughput of database T-RFLP permits much greater replication and may, therefore, be preferable for many ecological questions, particularly when combined with other techniques such as cloning.
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Registered trials
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