ArticleEuropean journal of applied physiology2007
Calpain/calpastatin activities and substrate depletion patterns during hindlimb unweighting and reweighting in skeletal muscle.
Article in European journal of applied physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Neuromuscular Electrical Stimulation to Combat Muscle Atrophy During Spaceflight: A Narrative Review of Mechanisms and Potential Applications.Life (Basel, Switzerland) · 2026Review
- Investigating Eukaryotic Elongation Factor 2 Kinase/Eukaryotic Translation Elongation Factor 2 Pathway Regulation and Its Role in Protein Synthesis Impairment during Disuse-Induced Skeletal Muscle Atrophy.The American journal of pathology · 2023Article
- Functional Nutrients to Ameliorate Neurogenic Muscle Atrophy.Metabolites · 2022Review
- Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.Pflugers Archiv : European journal of physiology · 2022Article
- Roles of ATP and SERCA in the Regulation of Calcium Turnover in Unloaded Skeletal Muscles: Current View and Future Directions.International journal of molecular sciences · 2022Review
- Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading.Doklady. Biochemistry and biophysics · 2020Article
- How Postural Muscle Senses Disuse? Early Signs and Signals.International journal of molecular sciences · 2020Review
- Effect of L-Arginine on Titin Expression in Rat Soleus Muscle After Hindlimb Unloading.Frontiers in physiology · 2019Article
- Cellular Responses of Human Postural Muscle to Dry Immersion.Frontiers in physiology · 2019Review
- Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- Human muscle signaling responses to 3-day head-out dry immersion.Journal of musculoskeletal & neuronal interactions · 2015Article
- Activity, abundance and expression of Ca²⁺-activated proteases in skeletal muscle of the aestivating frog, Cyclorana alboguttata.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2015Article
- Isoform composition and gene expression of thick and thin filament proteins in striated muscles of mice after 30-day space flight.BioMed research international · 2015Article
- Time course of diaphragm function recovery after controlled mechanical ventilation in rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Serological muscle loss biomarkers: an overview of current concepts and future possibilities.Journal of cachexia, sarcopenia and muscle · 2013Article
- Specific training improves skeletal muscle mitochondrial calcium homeostasis after eccentric exercise.European journal of applied physiology · 2013Article
- Cell mechanosensitivity: mechanical properties and interaction with gravitational field.BioMed research international · 2013Review
- Chronic hypobaric hypoxia mediated skeletal muscle atrophy: role of ubiquitin-proteasome pathway and calpains.Molecular and cellular biochemistry · 2012Article
- Structure and functional characteristics of rat's left ventricle cardiomyocytes under antiorthostatic suspension of various duration and subsequent reloading.Journal of biomedicine & biotechnology · 2012Article
- Various jobs of proteolytic enzymes in skeletal muscle during unloading: facts and speculations.Journal of biomedicine & biotechnology · 2012Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unloading of skeletal muscle by hindlimb unweighting (HU) is characterized by atrophy, protein loss, and an elevation in intracellular Ca(2+) levels that may be sufficient to activate Ca(2+)-dependent proteases (calpains). In this study, we investigated the time course of calpain activation and the depletion pattern of a specific structural protein (desmin) with unloading and subsequent reweighting. Rats underwent 12 h, 24 h, 72 h or 9 days of HU, followed by reweighting for either 0, 12 or 24 h. Total calpain-like activity was elevated with HU in skeletal muscle (P < 0.05) and was further enhanced with reweighting (P < 0.05). The increases in calpain-like activity were associated with a proportional increase in activity of the particulate fraction (P < 0.05). Activity of the mu-calpain isoform was elevated with 12 and 24 h of HU (P < 0.05) and returned to control levels thereafter. With reweighting, activities of mu-calpain were elevated above control levels for all HU groups except 9 days (P < 0.05). In contrast, minimal changes in m-calpain and calpastatin activity were observed with HU and reweighting. Although desmin depletion levels did not reach statistical significance, a significant inverse relationship was found between the mu-calpain/calpastatin ratio and the amount of desmin in isolated myofibrils (R = -0.83, P < 0.001). The results suggest that calpain activation is an early event during unloading in skeletal muscle, and that the majority of the increase in calpain activity can be attributed to the micro-isoform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.