Evidence map›Paper›PMID 17429572›Full record

ArticleExtremophiles : life under extreme conditions2007

Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp.

Yasushi Kageyama, Yoshihiro Takaki, Shigeru Shimamura, Shinro Nishi, Yuichi Nogi, Kohsuke Uchimura, Tohru Kobayashi, Jun Hitomi, Katsuya Ozaki, Shuji Kawai and 2 more

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Extremophiles : life under extreme conditions, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

  1. Metal Transformation by a NovelFrontiers in microbiology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yasushi KageyamaTochigi Research Laboratories of Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan. kageyama.yasushi@kao.co.jp
Yoshihiro Takaki
Shigeru Shimamura
Shinro Nishi
Yuichi Nogi
Kohsuke Uchimura
Tohru Kobayashi
Jun Hitomi
Katsuya Ozaki
Shuji Kawai
Susumu Ito
Koki Horikoshi
Japan Agency for Marine-Earth Science and Technology · JPKao Corporation (Japan) · JPHokkaido University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned 16S rRNA genes revealed two distinct types of variable region V1. Moreover, some cloned 16S rRNA genes in some of the reference strains of B. clausii had a V1 region of yet another type. The B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence. Bacillus sp. strain KSM-K16 was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B. clausii.

Indexed as

Genetic VariationRibotypingRNA, Ribosomal, 16SBacillusBase SequenceCloning, MolecularDNA, BacterialDNA, RibosomalElectrophoresis, Gel, Pulsed-FieldGenotypeMolecular Sequence DataNucleic Acid HybridizationOperonPhenotypePhylogenySequence Analysis, DNADNA, BacterialDNA, Ribosomalmicrobial serine proteinasesM-proteaseRNA, Ribosomal, 16SSerine EndopeptidasesSubtilisins

Identifiers

PMID17429572
OpenAlexW2123069084

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.