ArticleMolecular biology of the cell2007
Multiple interactions drive adaptor-mediated recruitment of the ubiquitin ligase rsp5 to membrane proteins in vivo and in vitro.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Nutritional Immunity and Fungal Pathogens: a New Role for Manganese.Current clinical microbiology reports · 2024Article
- α-Arrestins and Their Functions: From Yeast to Human Health.International journal of molecular sciences · 2022Review
- Adaptors as the regulators of HECT ubiquitin ligases.Cell death and differentiation · 2021Review
- A selective transmembrane recognition mechanism by a membrane-anchored ubiquitin ligase adaptor.The Journal of cell biology · 2021Article
- Endocytosis of nutrient transporters in fungi: The ART of connecting signaling and trafficking.Computational and structural biotechnology journal · 2021Review
- Genetic Analysis of Peroxisomal Genes Required for Longevity in a Yeast Model of Citrin Deficiency.Diseases (Basel, Switzerland) · 2020Article
- Overexpression of the peroxin Pex34p suppresses impaired acetate utilization in yeast lacking the mitochondrial aspartate/glutamate carrier Agc1p.FEMS yeast research · 2019Article
- Sodium Acetate Responses inFrontiers in microbiology · 2018Review
- Mechanism of ubiquitin chain synthesis employed by a HECT domain ubiquitin ligase.The Journal of biological chemistry · 2017Article
- Internalization of Heterologous Sugar Transporters by Endogenous α-Arrestins in the Yeast Saccharomyces cerevisiae.Applied and environmental microbiology · 2016Article
- The multidrug resistance pump ABCB1 is a substrate for the ubiquitin ligase NEDD4-1.Molecular membrane biology · 2015Article
- Manganese induces oligomerization to promote down-regulation of the intracellular trafficking receptor used by Shiga toxin.Molecular biology of the cell · 2014Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- Quality control: quality control at the plasma membrane: one mechanism does not fit all.The Journal of cell biology · 2014Review
- Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters.Frontiers in pharmacology · 2014Review
- Endocytic regulation of alkali metal transport proteins in mammals, yeast and plants.Current genetics · 2013Review
- Nedd4 is a specific E3 ubiquitin ligase for the NMDA receptor subunit GluN2D.Neuropharmacology · 2013Article
- SUMOylation regulates the homologous to E6-AP carboxyl terminus (HECT) ubiquitin ligase Rsp5p.The Journal of biological chemistry · 2013Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- The yeast p5 type ATPase, spf1, regulates manganese transport into the endoplasmic reticulum.PloS one · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Recognition of membrane proteins by the Nedd4/Rsp5 ubiquitin ligase family is a critical step in their targeting to the multivesicular body pathway. Some substrates contain "PY" motifs (PPxY), which bind to WW domains in the ligase. Others lack PY motifs and instead rely on adaptors that recruit the ligase to them. To investigate the mechanism of adaptor-mediated ubiquitination, we have characterized the interactions between the adaptor Bsd2, the ubiquitin ligase Rsp5, and the membrane proteins Cps1, Tre1, and Smf1 from Saccharomyces cerevisiae. We have reconstituted adaptor-mediated modification of Cps1 and Tre1 in vitro, and we show that two PY motifs in Bsd2 and two WW domains (WW2 and WW3) in Rsp5 are crucial for this. The binding of a weak noncanonical DMAPSY motif in Bsd2 to WW3 is an absolute requirement for Bsd2 adaptor function. We show that sorting of the manganese transporter Smf1, which requires both Bsd2 and Tre1, depends upon two PY motifs in Bsd2 and one motif in Tre1 but only two WW domains in Rsp5. We suggest that sequential assembly of first a Bsd2/Rsp5 complex, then a Tre1/Bsd2/Rsp5 complex followed by a rearrangement of PY-WW interactions is required for the ubiquitination of Smf1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.