ArticleMolecular biology of the cell2007
Cx43 mediates TGF-beta signaling through competitive Smads binding to microtubules.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 102 citations in OpenAlex.
- Cytoplasmic connexin43-microtubule interactions promote glioblastoma stem-like cell maintenance and tumorigenicity.Cell death & disease · 2025Article
- Skeletal abnormalities caused by a Connexin43Bone research · 2025Article
- Connexin-43 in Cancer: Above and Beyond Gap Junctions!Cancers · 2024Review
- Review
- Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress.Journal of vascular research · 2023Article
- Pharmacological inhibition of HDAC6 improves muscle phenotypes in dystrophin-deficient mice by downregulating TGF-β via Smad3 acetylation.Nature communications · 2022Article
- Connexin43 in Post-Surgical Peritoneal Adhesion Formation.Life (Basel, Switzerland) · 2022Article
- Cx43 upregulation in HUVECs under stretch via TGF-β1 and cytoskeletal network.Open medicine (Warsaw, Poland) · 2022Article
- Connecting different heart diseases through intercellular communication.Biology open · 2021Review
- Temozolomide Induces the Acquisition of Invasive Phenotype by O6-Methylguanine-DNA Methyltransferase (MGMT)International journal of molecular sciences · 2021Article
- Epidermal Growth Factor (EGF) Augments the Invasive Potential of Human Glioblastoma Multiforme Cells via the Activation of Collaborative EGFR/ROS-Dependent Signaling.International journal of molecular sciences · 2020Article
- Connexins in cancer: bridging the gap to the clinic.Oncogene · 2019Review
- TGF-β1 promotes gap junctions formation in chondrocytes via Smad3/Smad4 signalling.Cell proliferation · 2019Article
- Fibroblast-to-myofibroblast transition in bronchial asthma.Cellular and molecular life sciences : CMLS · 2018Review
- Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo.Nature communications · 2018Article
- Review
- Connexin Communication Compartments and Wound Repair in Epithelial Tissue.International journal of molecular sciences · 2018Review
- The Connexin Mimetic Peptide Gap27 and Cx43-Knockdown Reveal Differential Roles for Connexin43 in Wound Closure Events in Skin Model Systems.International journal of molecular sciences · 2018Article
- Mechanism of action of the anti-inflammatory connexin43 mimetic peptide JM2.American journal of physiology. Cell physiology · 2017Article
- Paracrine effect of CXCR4-overexpressing mesenchymal stem cells on ischemic heart injury.Cell biochemistry and function · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transforming growth factor-beta (TGF-beta) superfamily members play an important role in growth, differentiation, adhesion, apoptosis, and development in many species from insects and worms to vertebrates. Recently, TGF-beta signaling has been demonstrated to be negatively regulated by microtubules (MTs), which anchor endogenous Smad2/3 to cytosol and also directly interact with connexin43 (Cx43), and the activity of TGF-beta is mediated by Cx43. However, the mechanism underlying the intracellular regulation of TGF-beta activity by Cx43 remains unknown. Here, we found that the functional link between TGF-beta activation and Cx43 is mediated by interactions among Smad2/3, MTs, and Cx43. We confirmed that Cx43 competes with Smad2/3 for binding to MTs, which Cx43 specifically induces release of Smad2/3 from MTs and increases phospho-Smad2 and which, as a result, Smad2/3 and Smad4 are accumulated in the nucleus, leading to activation of the transcription of target genes. Consistently, knockdown of the endogenous Cx43 activity with double-strand RNA (dsRNA) in HL1 cardiomyocytes and Cx43 knockout mice cardiomyocytes consistently show the opposite effect. Our findings demonstrate a novel mechanism for Cx43 positive regulation of TGF-beta function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.