ArticleMolecular biology of the cell2007
Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Ccq1 restrains Mre11-mediated degradation to distinguish short telomeres from double-strand breaks.Nucleic acids research · 2024Article
- Improving homology-directed repair by small molecule agents for genetic engineering in unconventional yeast?-Learning from the engineering of mammalian systems.Microbial biotechnology · 2024Review
- Exploring Genetic Interactions with Telomere Protection GeneBiomolecules · 2023Review
- Gain-of-function mutations in RPA1 cause a syndrome with short telomeres and somatic genetic rescue.Blood · 2022Article
- Functional Diversification of Replication Protein A Paralogs and Telomere Length Maintenance in Arabidopsis.Genetics · 2020Article
- Homologous recombination repair intermediates promote efficient de novo telomere addition at DNA double-strand breaks.Nucleic acids research · 2020Article
- Review
- A Matter of Scale and Dimensions: Chromatin of Chromosome Landmarks in the Fungi.Microbiology spectrum · 2017Review
- 5' to 3' Unfolding Directionality of DNA Secondary Structures by Replication Protein A: G-QUADRUPLEXES AND DUPLEXES.The Journal of biological chemistry · 2016Article
- Managing Single-Stranded DNA during Replication Stress in Fission Yeast.Biomolecules · 2015Review
- RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.The EMBO journal · 2015Article
- Replication protein A: single-stranded DNA's first responder: dynamic DNA-interactions allow replication protein A to direct single-strand DNA intermediates into different pathways for synthesis or repair.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
- RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.The EMBO journal · 2012Article
- RPA and POT1: friends or foes at telomeres?Cell cycle (Georgetown, Tex.) · 2012Article
- The telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation.Molecular and cellular biology · 2011Article
- Break-induced ATR and Ddb1-Cul4(Cdt)² ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast.Genes & development · 2010Article
- Telomeres: structures in need of unwinding.FEBS letters · 2010Review
- Telomeric protein TRF2 protects Holliday junctions with telomeric arms from displacement by the Werner syndrome helicase.Nucleic acids research · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The telomere complex must allow nucleases and helicases to process chromosome ends to make them substrates for telomerase, while preventing these same activities from disrupting chromosome end-protection. Replication protein A (RPA) binds to single-stranded DNA and is required for DNA replication, recombination, repair, and telomere maintenance. In fission yeast, the telomere binding protein Taz1 protects telomeres and negatively regulates telomerase. Here, we show that taz1-d rad11-D223Y double mutants lose their telomeric DNA, indicating that RPA (Rad11) and Taz1 are synergistically required to prevent telomere loss. Telomere loss in the taz1-d rad11-D223Y double mutants was suppressed by additional mutation of the helicase domain in a RecQ helicase (Rqh1), or by overexpression of Pot1, a single-strand telomere binding protein that is essential for protection of chromosome ends. From our results, we propose that in the absence of Taz1 and functional RPA, Pot1 cannot function properly and the helicase activity of Rqh1 promotes telomere loss. Our results suggest that controlling the activity of Rqh1 at telomeres is critical for the prevention of genomic instability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.