ArticleJournal of clinical microbiology2007
Rapid and reliable method for quantification of Mycobacterium paratuberculosis by use of the BACTEC MGIT 960 system.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
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- Experimental evidence of the anti-bacterial activity pathway of copper ion treatment on Mycobacterium avium subsp. paratuberculosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
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- Ensemble density-dependent synchronization of mycobacterial growth: BACTEC MGIT 960 fluorescence-based analysis and mathematical modelling of coupled biophysical and chemical processes.AIMS microbiology · 2022Article
- Development of Human Cell-BasedFrontiers in cellular and infection microbiology · 2022Article
- Validation of a Novel Diagnostic Approach Combining the VersaTREK™ System for Recovery and Real-Time PCR for the Identification ofMicroorganisms · 2021Article
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- Transcriptome Profiling of Bovine Macrophages Infected byFrontiers in immunology · 2019Article
- In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions.BMC microbiology · 2018Article
- Quantifying intracellular Mycobacterium tuberculosis: An essential issue for in vitro assays.MicrobiologyOpen · 2018Review
- Case definition terminology for paratuberculosis (Johne's disease).BMC veterinary research · 2017Article
- Current status ofThe Indian journal of medical research · 2016Review
- A Rapid Method for Quantifying Viable Mycobacterium avium subsp. paratuberculosis in Cellular Infection Assays.Applied and environmental microbiology · 2016Article
- Article
- Time to detection of Mycobacterium tuberculosis using the MGIT 320 system correlates with colony counting in preclinical testing of new vaccines.Clinical and vaccine immunology : CVI · 2014Article
- Filtration improves the performance of a high-throughput screen for anti-mycobacterial compounds.PloS one · 2014Article
- Detection and confirmation of Mycobacterium avium subsp. paratuberculosis in direct quantitative PCR positive fecal samples by the manual fluorescent MGIT culture system.The Journal of veterinary medical science · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple method for the enumeration of viable Mycobacterium paratuberculosis cells was developed and evaluated using the MGIT 960 culture system. For each of 12 M. paratuberculosis strains isolated from either cattle or humans, single-cell suspensions of M. paratuberculosis cells were adjusted to an optical density at 600 nm of 1.00 (10(7.6) to 10(8.2) cells/ml), and serial dilutions were prepared. Standard curves were established by relating the MGIT time-to-detection data to the log10 CFU for these suspensions using standard plate counting and BACTEC 460 results as reference methods. Universal and strain-specific standard quantification curves were generated. A one-phase exponential decay equation best fit the universal standard curve and strain-specific curves (R2 of 0.96 and >0.99, respectively). Two subgroups within the universal curves were distinguished: one for laboratory-adapted strains and the other for recently isolated low-passage bovine strains. The predictive errors for log(10) estimations using the universal standard curve, each subgroup's standard curve, and strain-specific curves were +/-0.87, +/-0.45, and +/-0.31 log10 units, respectively. CFU estimations by all three standard curves were highly reproducible, regardless of the M. paratuberculosis strain or inoculum volume. In comparison with the previously described BACTEC 460 M. paratuberculosis counting method, quantification with MGIT 960 was less expensive, more rapid, more accurate, and more sensitive (<10 CFU). This MGIT counting method has broad applications for studies requiring the quantification of viable M. paratuberculosis cells, such as drug susceptibility testing or environmental survival studies.
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