ArticleBMC plant biology2007
Discovery of chemically induced mutations in rice by TILLING.
Article in BMC plant biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
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Who cites it
152 citing papers in PubMed, 458 citations in OpenAlex.
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- Genome-Wide Association Analyses Reveal the Genetic Basis of EMS Mutagenesis Efficiency in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Non-Random Distribution of EMS-Induced Mutations Reveals Preference for Open Chromatin and Expressed Genes in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Exploring the multifaceted dynamics of flowering time regulation in field crops: Insight and intervention approaches.The plant genome · 2025Review
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Induced genetic diversity through mutagenesis in wheat gene pool and significant use of SCoT markers to underpin key agronomic traits.BMC plant biology · 2024Article
- Finger millet: a hero in the making to combat food insecurity.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Plant Functional Genomics Based on High-Throughput CRISPR Library Knockout Screening: A Perspective.Advanced genetics (Hoboken, N.J.) · 2024Article
- Genomics of predictive radiation mutagenesis in oilseed rape: modifying seed oil composition.Plant biotechnology journal · 2024Article
- Rice mutants, selected under severe drought stress, show reduced stomatal density and improved water use efficiency under restricted water conditions.Frontiers in plant science · 2024Article
- Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Metabolites · 2023Article
- Expression of the wheat multipathogen resistance hexose transporter Lr67res is associated with anion fluxes.Plant physiology · 2023Article
- How Do Plants Cope with DNA Damage? A Concise Review on the DDR Pathway in Plants.International journal of molecular sciences · 2023Review
- Characterization of prolycopene-accumulated TanJournal of biosciences · 2023Article
- Augmenting tomato functional genomics with a genome-wide induced genetic variation resource.Frontiers in plant science · 2023Article
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Accelerated Domestication of New Crops: Yield is Key.Plant & cell physiology · 2022Article
- Construction and analysis of a Noccaea caerulescens TILLING population.BMC plant biology · 2022Article
- A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley.Plant communications · 2022Article
92 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRice is both a food source for a majority of the world's population and an important model system. Available functional genomics resources include targeted insertion mutagenesis and transgenic tools. While these can be powerful, a non-transgenic, unbiased targeted mutagenesis method that can generate a range of allele types would add considerably to the analysis of the rice genome. TILLING (Targeting Induced Local Lesions in Genomes), a general reverse genetic technique that combines traditional mutagenesis with high throughput methods for mutation discovery, is such a method.
resultsTo apply TILLING to rice, we developed two mutagenized rice populations. One population was developed by treatment with the chemical mutagen ethyl methanesulphonate (EMS), and the other with a combination of sodium azide plus methyl-nitrosourea (Az-MNU). To find induced mutations, target regions of 0.7-1.5 kilobases were PCR amplified using gene specific primers labeled with fluorescent dyes. Heteroduplexes were formed through denaturation and annealing of PCR products, mismatches digested with a crude preparation of CEL I nuclease and cleaved fragments visualized using denaturing polyacrylamide gel electrophoresis. In 10 target genes screened, we identified 27 nucleotide changes in the EMS-treated population and 30 in the Az-MNU population.
conclusionWe estimate that the density of induced mutations is two- to threefold higher than previously reported rice populations (about 1/300 kb). By comparison to other plants used in public TILLING services, we conclude that the populations described here would be suitable for use in a large scale TILLING project.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.