ArticleGenome biology2007
Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- HIRA directly targets the enhancers of selected cardiac transcription factors during in vitro differentiation of mouse embryonic stem cells.Molecular biology reports · 2018Article
- Murine Embryonic Stem Cell Plasticity Is Regulated through Klf5 and Maintained by Metalloproteinase MMP1 and Hypoxia.PloS one · 2016Article
- Fam40b is required for lineage commitment of murine embryonic stem cells.Cell death & disease · 2014Article
- Article
- Growth requirements and chromosomal instability of induced pluripotent stem cells generated from adult canine fibroblasts.Stem cells and development · 2013Article
- Downregulation of doxorubicin-induced myocardial apoptosis accompanies postnatal heart maturation.American journal of physiology. Heart and circulatory physiology · 2012Article
- Specific gene signatures and pathways in mesodermal cells and their derivatives derived from embryonic stem cells.Stem cell reviews and reports · 2012Review
- Functional characterization and gene expression profiling of α-smooth muscle actin expressing cardiomyocytes derived from murine induced pluripotent stem cells.Stem cell reviews and reports · 2012Article
- Distinct signaling properties of mitogen-activated protein kinase kinases 4 (MKK4) and 7 (MKK7) in embryonic stem cell (ESC) differentiation.The Journal of biological chemistry · 2012Article
- Derivation of es Cells From Early Stage Preimplantation Embryos and Characterisation of their Cardiac Differentiation Potential in Mice.Journal of stem cells & regenerative medicine · 2012Article
- Purification of cardiac cells from Drosophila embryos.Methods (San Diego, Calif.) · 2012Article
- Proceedings of the 6th annual meeting of the Germany society for stem cell research.Journal of stem cells & regenerative medicine · 2012Article
- Cytosine arabinoside induces ectoderm and inhibits mesoderm expression in human embryonic stem cells during multilineage differentiation.British journal of pharmacology · 2011Article
- Promises and pitfalls in cell replacement therapy for heart failure.Drug discovery today. Disease mechanisms · 2010Article
- The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation.PloS one · 2009Article
- Three LIF-dependent signatures and gene clusters with atypical expression profiles, identified by transcriptome studies in mouse ES cells and early derivatives.BMC genomics · 2009Article
- Beta-Catenin downregulation attenuates ischemic cardiac remodeling through enhanced resident precursor cell differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Micro-proteome analysis using micro-chromatofocusing in intact protein separations.Journal of chromatography. A · 2008Article
- Interactome of a cardiopoietic precursor.Journal of cardiovascular translational research · 2008Review
- Genomic chart guiding embryonic stem cell cardiopoiesis.Genome biology · 2008Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCharacterization of gene expression signatures for cardiomyocytes derived from embryonic stem cells will help to define their early biologic processes.
resultsA transgenic alpha-myosin heavy chain (MHC) embryonic stem cell lineage was generated, exhibiting puromycin resistance and expressing enhanced green fluorescent protein (EGFP) under the control of the alpha-MHC promoter. A puromycin-resistant, EGFP-positive, alpha-MHC-positive cardiomyocyte population was isolated with over 92% purity. RNA was isolated after electrophysiological characterization of the cardiomyocytes. Comprehensive transcriptome analysis of alpha-MHC-positive cardiomyocytes in comparison with undifferentiated alpha-MHC embryonic stem cells and the control population from 15-day-old embryoid bodies led to identification of 884 upregulated probe sets and 951 downregulated probe sets in alpha-MHC-positive cardiomyocytes. A subset of upregulated genes encodes cytoskeletal and voltage-dependent channel proteins, and proteins that participate in aerobic energy metabolism. Interestingly, mitosis, apoptosis, and Wnt signaling-associated genes were downregulated in the cardiomyocytes. In contrast, annotations for genes upregulated in the alpha-MHC-positive cardiomyocytes are enriched for the following Gene Ontology (GO) categories: enzyme-linked receptor protein signaling pathway (GO:0007167), protein kinase activity (GO:0004672), negative regulation of Wnt receptor signaling pathway (GO:0030178), and regulation of cell size (O:0008361). They were also enriched for the Biocarta p38 mitogen-activated protein kinase signaling pathway and Kyoto Encyclopedia of Genes and Genomes (KEGG) calcium signaling pathway.
conclusionThe specific pattern of gene expression in the cardiomyocytes derived from embryonic stem cells reflects the biologic, physiologic, and functional processes that take place in mature cardiomyocytes. Identification of cardiomyocyte-specific gene expression patterns and signaling pathways will contribute toward elucidating their roles in intact cardiac function.
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