ArticleAnalytical chemistry2007
Secondary ion MS imaging to relatively quantify cholesterol in the membranes of individual cells from differentially treated populations.
Article in Analytical chemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Improvements in high-yield detection and localisation of cholesterol in murine brains using water cluster beam secondary ion mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- ToF-SIMS Imaging for the Analysis of Cholesterol Formation at Macrophage Membrane.Metabolites · 2025Article
- Dysregulation of cellular membrane homeostasis as a crucial modulator of cancer risk.The FEBS journal · 2024Review
- The localization of the alkaloids inFrontiers in plant science · 2022Article
- Molecular insights into symbiosis-mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation.Analytical and bioanalytical chemistry · 2021Article
- Chemical Analysis of Single Cells and Organelles.Analytical chemistry · 2021Review
- Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles.ACS nano · 2020Article
- Multimodal, in Situ Imaging of Ex Vivo Human Skin Reveals Decrease of Cholesterol Sulfate in the Neoepithelium during Acute Wound Healing.Analytical chemistry · 2020Article
- Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique.Analytical chemistry · 2019Article
- Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells.Journal of visualized experiments : JoVE · 2019Article
- Review
- MALDI MS Guided Liquid Microjunction Extraction for Capillary Electrophoresis-Electrospray Ionization MS Analysis of Single Pancreatic Islet Cells.Analytical chemistry · 2017Article
- Visualization of metallodrugs in single cells by secondary ion mass spectrometry imaging.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Review
- Lithium Accumulates in Neurogenic Brain Regions as Revealed by High Resolution Ion Imaging.Scientific reports · 2017Article
- Using Dicationic Ion-Pairing Compounds To Enhance the Single Cell Mass Spectrometry Analysis Using the Single-Probe: A Microscale Sampling and Ionization Device.Analytical chemistry · 2016Article
- Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions.Journal of visualized experiments : JoVE · 2016Article
- Emerging mass spectrometry techniques for the direct analysis of microbial colonies.Current opinion in microbiology · 2014Article
- Imaging mass spectrometry in neuroscience.ACS chemical neuroscience · 2013Review
- Mass spectrometry imaging and profiling of single cells.Journal of proteomics · 2012Review
- Identifying individual cell types in heterogeneous cultures using secondary ion mass spectrometry imaging with C60 etching and multivariate analysis.Analytical chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a well-established bioanalytical method for directly imaging the chemical distribution across single cells. Here we report a protocol for the use of SIMS imaging to comparatively quantify the relative difference in cholesterol level between the plasma membranes of two cells. It should be possible to apply this procedure to the study of other selected lipids. This development enables direct comparison of the chemical effects of different drug treatments and incubation conditions in the plasma membrane at the single-cell level. Relative, quantitative TOF-SIMS imaging has been used here to compare macrophage cells treated to contain elevated levels of cholesterol with respect to control cells. In situ fluorescence microscopy with two different membrane dyes was used to discriminate morphologically similar but differentially treated cells prior to SIMS analysis. SIMS images of fluorescently identified cells reveal that the two populations of cells have distinct outer leaflet membrane compositions with the membranes of the cholesterol-treated macrophages containing more than twice the amount of cholesterol of control macrophages. Relative quantification with SIMS to compare the chemical composition of single cells can provide valuable information about normal biological functions, causative agents of diseases, and possible therapies for diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.