ArticlePlant molecular biology2007
The Arabidopsis thaliana GSK3/Shaggy like kinase AtSK3-2 modulates floral cell expansion.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- PagKNAT5a promotes plant growth by enhancing xylem cell elongation and secondary wall formation in poplar.Horticulture research · 2025Article
- Article
- PHYTOCHROME-INTERACTING FACTOR 7 and RELATIVE OF EARLY FLOWERING 6 act in shade avoidance memory in Arabidopsis.Nature communications · 2024Article
- Brassinosteroid biosynthesis and signaling: Conserved and diversified functions of core genes across multiple plant species.Plant communications · 2024Review
- Genome-Wide Association Study and Prediction of Tassel Weight of Tropical Maize Germplasm in Multi-Parent Population.International journal of molecular sciences · 2024Article
- A Phytochrome B-PIF4-MYC2/MYC4 module inhibits secondary cell wall thickening in response to shaded light.Plant communications · 2022Article
- Ubiquitinated DA1 negatively regulates vascular cambium activity through modulating the stability of WOX4 in Populus.The Plant cell · 2022Article
- Genome-wide identification and expression analysis of the GSK gene family in wheat (Triticum aestivum L.).Molecular biology reports · 2022Article
- Virus-induced gene silencing (VIGS) analysis shows involvement of the LsSTPK gene in lettuce (Plant signaling & behavior · 2021Article
- SHAGGY-like kinase 12 regulates flowering through mediating CONSTANS stability inScience advances · 2020Article
- Genome-wide characterization and phylogenetic analysis of GSK gene family in three species of cotton: evidence for a role of some GSKs in fiber development and responses to stress.BMC plant biology · 2018Article
- Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Horticulture research · 2018Article
- Annotation and profiling of barley GLYCOGEN SYNTHASE3/Shaggy-like genes indicated shift in organ-preferential expression.PloS one · 2018Article
- The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins.Plant molecular biology · 2016Article
- Suppression of ASKβ (AtSK32), a Clade III Arabidopsis GSK3, Leads to the Pollen Defect during Late Pollen Development.Molecules and cells · 2015Article
- The Utilization of Plant Facilities on the International Space Station-The Composition, Growth, and Development of Plant Cell Walls under Microgravity Conditions.Plants (Basel, Switzerland) · 2015Review
- Gene expression changes in Arabidopsis seedlings during short- to long-term exposure to 3-D clinorotation.Planta · 2011Article
- Expression analysis of sugarcane shaggy-like kinase (SuSK) gene identified through cDNA subtractive hybridization in sugarcane (Saccharum officinarum L.).Protoplasma · 2011Article
- A direct docking mechanism for a plant GSK3-like kinase to phosphorylate its substrates.The Journal of biological chemistry · 2010Article
- ASKtheta, a group-III Arabidopsis GSK3, functions in the brassinosteroid signalling pathway.The Plant journal : for cell and molecular biology · 2010Article
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Authors and funding
3 authors.
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Abstract
The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314]. In Arabidopsis thaliana, out of the 10 members of the GSK3/Shaggy-like gene family (AtSKs), a biological function has been assigned to only 1 member (AtSK2-1) by mutation. In the present work, a study was undertaken to elucidate the function of AtSK3-2. We have generated mutated versions of the A. thaliana Shaggy-like kinase 3-2 (AtSK3-2), in which Lys(167) and Arg(178), respectively homologues to Lys(85) and Arg(96) of the mammal GSK3beta, were modified into Ala by site-directed mutagenesis. In vitro kinase activity assays of the mutated recombinant protein AtSK3-2-R178A showed that the "primed activity" of the mutated kinase was reduced by 90% while the "non-primed" activity was only 20% reduced compared to the wild-type protein kinase. However, the mutant protein AtSK3-2-K167A showed no activity. Arabidopsis transgenic lines over-expressing AtSK3-2-R178A displayed smaller floral organs, namely pedicels, sepals and petals. Conversely, over-expression of both the wild-type AtSK3-2 protein and the AtSK3-2-K167A mutated version, displayed no altered morphogenesis. Scanning electron microscopic analyses of the AtSK3-2-R178A transgenic plants clearly showed a reduced cell size in flower organs, in which quantitative RT-PCR expression analyses of cell wall expansion enzymes showed reduced transcript levels of three xyloglucan endotransglycosylases (XET), namely XTH22 (TCH4), XTH23 (XTR6) and XTH30 (XTR4). Our data show that AtSK3-2 plays an important role in the control of cell elongation in flower development.
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