ArticleJournal of plant research2007
Disruption of the psbA gene by the copy correction mechanism reveals that the expression of plastid-encoded genes is regulated by photosynthesis activity.
Article in Journal of plant research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
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- Lighting Direction Affects Leaf Morphology, Stomatal Characteristics, and Physiology of Head Lettuce (International journal of molecular sciences · 2021Article
- Effects of light spectrum on morpho-physiological traits of grafted tomato seedlings.PloS one · 2021Article
- microRNA-dependent gene regulatory networks in maize leaf senescence.BMC plant biology · 2016Article
- Metabolic engineering of the chloroplast genome reveals that the yeast ArDH gene confers enhanced tolerance to salinity and drought in plants.Frontiers in plant science · 2015Article
- cDNA-AFLP analysis reveals the adaptive responses of citrus to long-term boron-toxicity.BMC plant biology · 2014Article
- Chloroplast-encoded chlB gene from Pinus thunbergii promotes root and early chlorophyll pigment development in Nicotiana tabaccum.Molecular biology reports · 2012Article
- Synthesis and expression of recombinant interferon alpha-5 gene in tobacco chloroplasts, a non-edible plant.Molecular biology reports · 2012Article
- Genome-wide transcriptome and proteome analyses of tobacco psaA and psbA deletion mutants.Plant molecular biology · 2011Article
- Extensive homologous recombination between introduced and native regulatory plastid DNA elements in transplastomic plants.Transgenic research · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functional analysis of genes encoded by the chloroplast genome of tobacco by reverse genetics is routine. Nevertheless, for a small number of genes their deletion generates heteroplasmic genotypes, complicating their analysis. There is thus the need for additional strategies to develop deletion mutants for these genes. We have developed a homologous copy correction-based strategy for deleting/mutating genes encoded on the chloroplast genome. This system was used to produce psbA knockouts. The resulting plants are homoplasmic and lack photosystem II (PSII) activity. Further, the deletion mutants exhibit a distinct phenotype; young leaves are green, whereas older leaves are bleached, irrespective of light conditions. This suggests that senescence is promoted by the absence of psbA. Analysis of the transcript levels indicates that NEP (nuclear-encoded plastid RNA polymerase)-dependent plastid genes are up regulated in the psbA deletion mutants, whereas the bleached leaves retain plastid-encoded plastid RNA polymerase activity. Hence, the expression of NEP-dependent plastid genes may be regulated by photosynthesis, either directly or indirectly.
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