ArticleNucleic acids research2007
A hybrid CMV-H1 construct improves efficiency of PEI-delivered shRNA in the mouse brain.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Thyroid hormone action in adult neurogliogenic niches: the known and unknown.Frontiers in endocrinology · 2024Review
- Thyroid hormone regulation of neural stem cell fate: From development to ageing.Acta physiologica (Oxford, England) · 2020Review
- Article
- Impaired oxidative endoplasmic reticulum stress response caused by deficiency of thyroid hormone receptor α.The Journal of biological chemistry · 2014Article
- Thyroid hormone signaling and adult neurogenesis in mammals.Frontiers in endocrinology · 2014Review
- A linear polyethylenimine mediated siRNA-based therapy targeting human epidermal growth factor receptor in SPC-A1 xenograft mice.Translational respiratory medicine · 2013Article
- Inhibition of Sox2 Expression in the Adult Neural Stem Cell Niche In Vivo by Monocationic-based siRNA Delivery.Molecular therapy. Nucleic acids · 2013Article
- Pseudotyped adeno-associated virus 2/9-delivered CCL11 shRNA alleviates lung inflammation in an allergen-sensitized mouse model.Human gene therapy · 2012Article
- Article
- Thyroid hormone potentiates insulin signaling and attenuates hyperglycemia and insulin resistance in a mouse model of type 2 diabetes.British journal of pharmacology · 2011Article
- Nonviral gene transfer as a tool for studying transcription regulation of xenobiotic metabolizing enzymes.Advanced drug delivery reviews · 2010Review
- Critical design criteria for minimal antibiotic-free plasmid vectors necessary to combine robust RNA Pol II and Pol III-mediated eukaryotic expression with high bacterial production yields.The journal of gene medicine · 2010Article
- Regulation of U6 promoter activity by transcriptional interference in viral vector-based RNAi.Genomics, proteomics & bioinformatics · 2010Article
- Liganded thyroid hormone receptor-alpha enhances proliferation of pancreatic beta-cells.The Journal of biological chemistry · 2010Article
- Thyroid hormone exerts negative feedback on hypothalamic type 4 melanocortin receptor expression.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Systemic delivery of DNA or siRNA mediated by linear polyethylenimine (L-PEI) does not induce an inflammatory response.Pharmaceutical research · 2008Article
- RNA interference for improving the outcome of islet transplantation.Advanced drug delivery reviewsReview
- Hybrid cytomegalovirus-U6 promoter-based plasmid vectors improve efficiency of RNA interference in zebrafish.Marine biotechnology (New York, N.Y.)Article
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-interference-driven loss of function in specific tissues in vivo should permit analysis of gene function in temporally and spatially defined contexts. However, delivery of efficient short hairpin RNA (shRNA) to target tissues in vivo remains problematic. Here, we demonstrate that efficiency of polyethylenimine (PEI)-delivered shRNA depends on the regulatory sequences used, both in vivo and in vitro. When tested in vivo, silencing of a luciferase target gene by shRNA produced from a hybrid construct composed of the CMV enhancer/promoter placed immediately upstream of an H1 promoter (50%) exceeds that obtained with the H1 promoter alone (20%). In contrast, in NIH 3T3 cells, the H1 promoter was more efficient than the hybrid construct (75 versus 60% inhibition of target gene expression, respectively). To test CMV-H1 shRNA efficiency against an endogenous gene in vivo, we used shRNA against thyroid hormone receptor alpha1 (TRalpha1). When vectorized in the mouse brain, the hybrid construct strongly derepressed CyclinD1-luciferase reporter gene expression, CyclinD1 being a negatively regulated thyroid hormone target gene. We conclude that promoter choice affects shRNA efficiency distinctly in different in vitro and in vivo situations and that a hybrid CMV-H1 construct is optimal for shRNA delivery in the mouse brain.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.