ArticleBMC microbiology2007
The current MLVA typing scheme for Enterococcus faecium is less discriminatory than MLST and PFGE for epidemic-virulent, hospital-adapted clonal types.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed.
- An epidemiological and molecular study regarding the spread of vancomycin-resistant Enterococcus faecium in a teaching hospital in Bogotá, Colombia 2016.BMC infectious diseases · 2019Article
- Invasive enterococcal infections in Poland: the current epidemiological situation.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Core Genome Multilocus Sequence Typing Scheme for High- Resolution Typing of Enterococcus faecium.Journal of clinical microbiology · 2015Article
- Random Amplified Polymorphic DNA (RAPD) Typing of Multidrug Resistant Enterococcus faecium Urinary Isolates from a Tertiary Care Centre, Northern India.Journal of clinical and diagnostic research : JCDR · 2013Article
- Dynamics of ampicillin-resistant Enterococcus faecium clones colonizing hospitalized patients: data from a prospective observational study.BMC infectious diseases · 2012Article
- Genomic diversification of enterococci in hosts: the role of the mobilome.Frontiers in microbiology · 2012Article
- Enterococcus faecium of the vanA genotype in rural drinking water, effluent, and the aqueous environment.Applied and environmental microbiology · 2012Article
- Intra- and interspecies genomic transfer of the Enterococcus faecalis pathogenicity island.PloS one · 2011Article
- Tn1546 is part of a larger plasmid-encoded genetic unit horizontally disseminated among clonal Enterococcus faecium lineages.The Journal of antimicrobial chemotherapy · 2010Article
- Global spread of the hyl(Efm) colonization-virulence gene in megaplasmids of the Enterococcus faecium CC17 polyclonal subcluster.Antimicrobial agents and chemotherapy · 2010Article
- Dogs are a reservoir of ampicillin-resistant Enterococcus faecium lineages associated with human infections.Applied and environmental microbiology · 2009Article
- A simple scoring system to differentiate between relapse and re-infection in patients with recurrent melioidosis.PLoS neglected tropical diseases · 2008Article
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Abstract
backgroundMLVA (multiple-locus variable-number tandem repeat analysis) is a reliable typing technique introduced recently to differentiate also isolates of Enterococcus faecium. We used the established VNTR (variable number of tandem repeats) scheme to test its suitability to differentiate 58 E. faecium isolates representing mainly outbreaks and clusters of infections and colonizations among patients from 31 German hospitals. All isolates were vancomycin-resistant (vanA type). Typing results for MLVA are compared with results of macrorestriction analysis in PFGE (pulsed-field gel electrophoresis) and MLST (multi-locus sequence typing).
resultsAll 51 but one hospital isolates from 1996-2006 were assigned to the clonal complex (CC) of epidemic-virulent, hospital-adapted lineages (MLST CC-17; MLVA CC-1) and differed from isolates of sporadic infections and colonizations (n = 7; 1991-1995) and other non-hospital origins (n = 27). Typing of all 58 hospital VRE revealed MLVA as the least discriminatory method (Simpson's diversity index 0.847) when compared to MLST (0.911) and PFGE (0.976). The two most common MLVA types MT-1 (n = 16) and MT-159 (n = 14) combined isolates of several MLST types including also major epidemic, hospital-adapted, clonal types (MT-1: ST-17, ST-18, ST-280, ST-282; MT-159: ST-78, ST-192, ST-203). These data clearly indicate that non-related E. faecium could possess an identical MLVA type being especially critical when MLVA is used to elucidate supposed outbreaks with E. faecium within a single or among different hospitals. Stability of a given MLVA profile MT-12 (ST-117) during an outbreak over a period of five years was also shown.
conclusionMLVA is a suitable method to assign isolates of E. faecium into distinct clonal complexes. To investigate outbreaks the current MLVA typing scheme for E. faecium does not discriminate enough and cannot be recommended as a standard superior to PFGE.
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