ArticleProceedings of the National Academy of Sciences of the United States of America2007
Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 68 citations in OpenAlex.
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- Light-induced oxidant production by fluorescent proteins.Free radical biology & medicine · 2018Review
- Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation.BMC biology · 2018Article
- Tyrosine dephosphorylated cortactin downregulates contractility at the epithelial zonula adherens through SRGAP1.Nature communications · 2017Article
- Subcellular Redox Targeting: Bridging in Vitro and in Vivo Chemical Biology.ACS chemical biology · 2017Review
- Model of turnover kinetics in the lamellipodium: implications of slow- and fast- diffusing capping protein and Arp2/3 complex.Physical biology · 2016Article
- An RPTPα/Src family kinase/Rap1 signaling module recruits myosin IIB to support contractile tension at apical E-cadherin junctions.Molecular biology of the cell · 2015Article
- Broadening the spectrum of actin-based protrusive activity mediated by Arp2/3 complex-facilitated polymerization: motility of cytoplasmic ridges and tubular projections.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Instantaneous inactivation of cofilin reveals its function of F-actin disassembly in lamellipodia.Molecular biology of the cell · 2013Article
- CD2AP links cortactin and capping protein at the cell periphery to facilitate formation of lamellipodia.Molecular and cellular biology · 2013Article
- Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin.Intravital · 2013Article
- Rab6a/a' are important Golgi regulators of pro-inflammatory TNF secretion in macrophages.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise spatiotemporal control of protein inactivation, enabling better understanding of the protein's role in cell function. EGFP has been used effectively as a CALI chromophore, and its cotranslational attachment to the target protein avoids having to use exogenously added labeling reagents. A potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the endogenous, unlabeled protein that is not susceptible to light inactivation. Performing EGFP-CALI experiments in deficient cells rescued with functional EGFP-fusion proteins permits more complete loss of function to be achieved. Here, we present a modified lentiviral system for rapid and efficient generation of knockdown cell lines complemented with physiological levels of EGFP-fusion proteins. We demonstrate that CALI of EGFP-CapZbeta increases uncapped actin filaments, resulting in enhanced filament growth and the formation of numerous protrusive structures. We show that these effects are completely dependent upon knocking down the endogenous protein. We also demonstrate that CALI of EGFP-Mena in Mena/VASP-deficient cells stabilizes lamellipodial protrusions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.