ArticleProceedings of the National Academy of Sciences of the United States of America2007
A mechanism for transcriptional repression dependent on the BRCA1 E3 ubiquitin ligase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- DNA damage repair factor Rad18 controls virulence partially via transcriptional suppression of genesVirulence · 2024Article
- The BRCA1/BARD1 ubiquitin ligase and its substrates.The Biochemical journal · 2021Review
- WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling.Nature communications · 2019Article
- Article
- Review
- BRCA1-Dependent Transcriptional Regulation: Implication in Tissue-Specific Tumor Suppression.Cancers · 2018Review
- Selective mode of action of plumbagin through BRCA1 deficient breast cancer stem cells.BMC cancer · 2016Article
- Dysregulation of ubiquitin ligases in cancer.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2015Review
- A design principle underlying the paradoxical roles of E3 ubiquitin ligases.Scientific reports · 2014Article
- BRCA1 and Oxidative Stress.Cancers · 2014Article
- BRCA1 targets G2/M cell cycle proteins for ubiquitination and proteasomal degradation.Oncogene · 2013Article
- Ubiquitination of retinoblastoma family protein 1 potentiates gene-specific repression function.The Journal of biological chemistry · 2012Article
- The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.The Journal of biological chemistry · 2012Article
- Promoters active in interphase are bookmarked during mitosis by ubiquitination.Nucleic acids research · 2012Article
- Links between genome integrity and BRCA1 tumor suppression.Trends in biochemical sciences · 2012Review
- Substitution of aspartic acid with glutamic acid at position 67 of the BRCA1 RING domain retains ubiquitin ligase activity and zinc(II) binding with a reduced transition temperature.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2011Article
- BRCA1/BARD1 complex interacts with steroidogenic factor 1--A potential mechanism for regulation of aromatase expression by BRCA1.The Journal of steroid biochemistry and molecular biology · 2011Article
- BRCA1 represses amphiregulin gene expression.Cancer research · 2010Article
- Transcriptional autoregulation by BRCA1.Cancer research · 2010Article
- BRCA1, hormone, and tissue-specific tumor suppression.International journal of biological sciences · 2009Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Loss of function of the tumor suppressor protein BRCA1 is responsible for a high percentage of familial and also sporadic breast cancers. Early work identified a stimulatory transcriptional coactivator function for the BRCA1 protein, and more recently, BRCA1 has been implicated in transcriptional repression, although few examples of repressed genes have been characterized. We recently used an in vitro transcription assay to identify a biochemical mechanism that explained the BRCA1 stimulatory activity. In this study, we identified an ubiquitin-dependent mechanism by which BRCA1 inhibits transcription. BRCA1 ubiquitinates the transcriptional preinitiation complex, preventing stable association of TFIIE and TFIIH, and thus blocks the initiation of mRNA synthesis. What is striking about this mechanism of regulation by BRCA1 is that the ubiquitination of the preinitiation complex is not targeting proteins for degradation by the proteasome, nor are ubiquitin receptors modifying the activity, but rather the ubiquitin moiety itself interferes with the assembly of basal transcription factors at the promoter. Using RNAi to knockdown expression of the endogenous BRCA1 protein, we assessed the level of repression dependent on BRCA1 in the cell, and we found that BRCA1 is at least as significant a transcriptional repressor as it is an activator. These results define a biochemical mechanism by which the BRCA1 enzymatic activity regulates a key cellular process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.