ArticleProceedings of the National Academy of Sciences of the United States of America2007
Determining the transgene containment level provided by chloroplast transformation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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103 citing papers in PubMed.
- Monitoring the fate of paternal mitochondria and their elimination in rice zygotes.Plant physiology · 2026Article
- The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.Plants (Basel, Switzerland) · 2026Review
- Review
- High-frequency biparental inheritance of plant mitochondria upon chilling stress and loss of a genome-degrading nuclease.Nature plants · 2026Article
- Risk assessment of plant-to-bacterium transgene flow associated with novel small synthetic genome (minisynplastome) platforms for plastid genetic engineering.FEMS microbiology ecology · 2026Article
- Biparental plastid inheritance and its implications in plastid genome engineering.Plant cell reports · 2025Review
- Development of chloroplast transformation for five species in the genus Nicotiana.The Plant journal : for cell and molecular biology · 2025Article
- Reverse genetics in the Arabidopsis chloroplast genome identifies rps16 as a transcribed pseudogene.The Plant journal : for cell and molecular biology · 2025Article
- Cytoplasmic inheritance: The transmission of plastid and mitochondrial genomes across cells and generations.Plant physiology · 2025Review
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases inPeerJ · 2025Article
- Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).Plant cell reports · 2024Article
- Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.ACS synthetic biology · 2024Article
- Characterization of organelle DNA degradation mediated by DPD1 exonuclease in the rice genome-edited line.Plant molecular biology · 2024Article
- Chromatography affinity resin with photosynthetically-sourced protein A ligand.Scientific reports · 2024Article
- Genetic characteristics of the diploid offsprings in potato Cooperation 88 induced by diploid donor IVP101.Frontiers in plant science · 2024Article
- An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression.Nucleic acids research · 2023Article
- Chloroplast Genomes and Phylogenetic Analysis of ThreeInternational journal of molecular sciences · 2023Article
- A Comprehensive Analysis of Chloroplast Genome Provides New Insights into the Evolution of the GenusInternational journal of molecular sciences · 2023Article
- Plastid engineering using episomal DNA.Plant cell reports · 2023Review
43 more citing papers are in PubMed but not listed here.
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Plastids (chloroplasts) are maternally inherited in most crops. Maternal inheritance excludes plastid genes and transgenes from pollen transmission. Therefore, plastid transformation is considered a superb tool for ensuring transgene containment and improving the biosafety of transgenic plants. Here, we have assessed the strictness of maternal inheritance and the extent to which plastid transformation technology confers an increase in transgene confinement. We describe an experimental system facilitating stringent selection for occasional paternal plastid transmission. In a large screen, we detected low-level paternal inheritance of transgenic plastids in tobacco. Whereas the frequency of transmission into the cotyledons of F(1) seedlings was approximately 1.58 x 10(-5) (on 100% cross-fertilization), transmission into the shoot apical meristem was significantly lower (2.86 x 10(-6)). Our data demonstrate that plastid transformation provides an effective tool to increase the biosafety of transgenic plants. However, in cases where pollen transmission must be prevented altogether, stacking with other containment methods will be necessary to eliminate the residual outcrossing risk.
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