ArticleProceedings of the National Academy of Sciences of the United States of America2007
Direct and adaptor-mediated substrate recognition by an essential AAA+ protease.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 88 citations in OpenAlex.
- A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1.PLoS genetics · 2025Article
- Structural insights into the Pseudomonas aeruginosa ClpP1•ClpP2 heterocomplex and its interactions with the AAA+ ClpX unfoldase.Protein science : a publication of the Protein Society · 2025Article
- Regulation of the transcription factor CdnL promotes adaptation to nutrient stress inPNAS nexus · 2024Article
- Diverse nature of ClpX degradation motifs inMicrobiology spectrum · 2024Article
- Regulation of the transcription factor CdnL promotes adaptation to nutrient stress inbioRxiv : the preprint server for biology · 2023Article
- ATP hydrolysis tunes specificity of a AAA+ protease.Cell reports · 2022Article
- Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.Cell reports · 2022Article
- Recent structural insights into the mechanism of ClpP protease regulation by AAA+ chaperones and small molecules.The Journal of biological chemistry · 2022Review
- ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.Journal of bacteriology · 2022Article
- DnaJ and ClpX Are Required for HitRS and HssRS Two-Component System Signaling in Bacillus anthracis.Infection and immunity · 2022Article
- Activation of a signaling pathway by the physical translocation of a chromosome.Developmental cell · 2021Article
- ClpP1P2 peptidase activity promotes biofilm formation in Pseudomonas aeruginosa.Molecular microbiology · 2021Article
- The Cleavage Profile of Protein Substrates by ClpXP Reveals Deliberate Starts and Pauses.Biochemistry · 2020Article
- Polar Localization Hub Protein PopZ Restrains Adaptor-Dependent ClpXP Proteolysis in Caulobacter crescentus.Journal of bacteriology · 2018Article
- Regulated Proteolysis in Bacteria.Annual review of biochemistry · 2018Review
- The C-Terminal Region of Bacillus subtilis SwrA Is Required for Activity and Adaptor-Dependent LonA Proteolysis.Journal of bacteriology · 2018Article
- Cargo engagement protects protease adaptors from degradation in a substrate-specific manner.The Journal of biological chemistry · 2017Article
- HspQ Functions as a Unique Specificity-Enhancing Factor for the AAA+ Lon Protease.Molecular cell · 2017Article
- Caulobacter crescentus CdnL is a non-essential RNA polymerase-binding protein whose depletion impairs normal growth and rRNA transcription.Scientific reports · 2017Article
- Two Isoforms of Clp Peptidase in Pseudomonas aeruginosa Control Distinct Aspects of Cellular Physiology.Journal of bacteriology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Regulated proteolysis is required to execute many cellular programs. In Caulobacter crescentus, timely degradation of the master regulator CtrA by ClpXP protease is essential for cell-cycle progression and requires the colocalization of CtrA and RcdA. Here, we establish a biochemical framework to understand regulated proteolysis in C. crescentus and show that RcdA is not an adaptor for CtrA degradation. CtrA is rapidly degraded without RcdA and is recognized with an affinity comparable with the best ClpXP substrates. In contrast, SspBalpha, the alpha-proteobacterial homolog of SspB, functions as an adaptor to enhance degradation of specific substrates. Cargo-free SspBalpha is also itself a substrate of ClpXP-mediated proteolysis. Thus, our analysis (i) reveals the consequences of both direct and adaptor-stimulated recognition in mediating substrate specificity in vitro, (ii) reveals a potential regulatory role of controlled adaptor stability, and (iii) suggests that cell-cycle regulation of CtrA stability depends on repression of its intrinsic degradation rather than adaptor-mediated enhancement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.