ArticleDevelopmental cell2007
Membrane hemifusion is a stable intermediate of exocytosis.
Article in Developmental cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- The Hidden Cost of Fusion: Intrinsic Asymmetry in Vesicle Fusion Restricts Synthetic Cell Growth.bioRxiv : the preprint server for biology · 2025Article
- SNARE Proteins in Synaptic Vesicle Fusion.Advances in neurobiology · 2023Article
- Reduction of focal sweating by lipid nanoparticle-delivered myricetin.Scientific reports · 2020Article
- SNAP23 is required for constitutive and regulated exocytosis in mouse oocytes†.Biology of reproduction · 2019Article
- Synaptic Vesicles Having Large Contact Areas with the Presynaptic Membrane are Preferentially Hemifused at Active Zones of Frog Neuromuscular Junctions Fixed during Synaptic Activity.International journal of molecular sciences · 2019Review
- Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.Biophysical journal · 2019Article
- Visualization of SNARE-Mediated Hemifusion between Giant Unilamellar Vesicles Arrested by Myricetin.Frontiers in molecular neuroscience · 2017Article
- Hemifusion in Synaptic Vesicle Cycle.Frontiers in molecular neuroscience · 2017Review
- Article
- A Chemical Controller of SNARE-Driven Membrane Fusion That Primes Vesicles for Ca(2+)-Triggered Millisecond Exocytosis.Journal of the American Chemical Society · 2016Article
- High Transmembrane Voltage Raised by Close Contact Initiates Fusion Pore.Frontiers in molecular neuroscience · 2016Article
- SNARE zippering is hindered by polyphenols in the neuron.Biochemical and biophysical research communications · 2014Article
- Transorganellar complementation redefines the biochemical continuity of endoplasmic reticulum and chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Evolution of the hemifused intermediate on the pathway to membrane fusion.Biophysical journal · 2012Article
- Membrane fusion intermediates via directional and full assembly of the SNARE complex.Science (New York, N.Y.) · 2012Article
- Energetics of stalk intermediates in membrane fusion are controlled by lipid composition.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Contribution of cryoelectron microscopy of vitreous sections to the understanding of biological membrane structure.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The SNARE complex in neuronal and sensory cells.Molecular and cellular neurosciences · 2012Review
- Zinc sparks are triggered by fertilization and facilitate cell cycle resumption in mammalian eggs.ACS chemical biology · 2011Article
- Palaeontological evidence of membrane relationship in step-by-step membrane fusion.Molecular membrane biology · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Membrane fusion during exocytosis requires that two initially distinct bilayers pass through a hemifused intermediate in which the proximal monolayers are shared. Passage through this intermediate is an essential step in the process of secretion, but is difficult to observe directly in vivo. Here we study membrane fusion in the sea urchin egg, in which thousands of homogeneous cortical granules are associated with the plasma membrane prior to fertilization. Using fluorescence redistribution after photobleaching, we find that these granules are stably hemifused to the plasma membrane, sharing a cytoplasmic-facing monolayer. Furthermore, we find that the proteins implicated in the fusion process-the vesicle-associated proteins VAMP/synaptobrevin, synaptotagmin, and Rab3-are each immobile within the granule membrane. Thus, these secretory granules are tethered to their target plasma membrane by a static, catalytic fusion complex that maintains a hemifused membrane intermediate.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.