ArticleGenesis (New York, N.Y. : 2000)2007
Enhanced transgenesis by intracytoplasmic injection of envelope-free lentivirus.
Article in Genesis (New York, N.Y. : 2000), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 17 citations in OpenAlex.
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- Long-term survival, axonal growth-promotion, and myelination of Schwann cells grafted into contused spinal cord in adult rats.Experimental neurology · 2014Article
- Production of germline transgenic prairie voles (Microtus ochrogaster) using lentiviral vectors.Biology of reproduction · 2009Article
- Generation of transgenic monkeys with human inherited genetic disease.Methods (San Diego, Calif.) · 2009Review
- Development of a novel transgenic rat overexpressing the P2Y(2) nucleotide receptor using a lentiviral vector.Journal of vascular research · 2009Article
- Lentiviral integration preferences in transgenic mice.Genesis (New York, N.Y. : 2000) · 2008Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate enhanced transgenesis in mice by intracytoplasmic injection of envelope-free lentivirus. Envelope-free lentivirus carrying the green fluorescent protein (GFP) gene under the control of the ubiquitin promoter (LVU-GFP) was microinjected into the cytoplasm of mouse zygotes prior to embryo transfer. Ninety-seven percent (31/32) of the adult mice were confirmed transgenic by PCR and Southern blot analysis; all founder mice express GFP when tail snips were examined by fluorescent microscopy prior to genomic DNA extraction. Transgene insertion numbers ranging from 1 to 32 were revealed by Southern blot analysis. Germline transmission was confirmed by the presence of transgene in F1 offspring. As expected, a lower transgenic rate (2.2%; 1/46) resulted when envelope-free LVU-GFP was microinjected into the perivitelline space (PVS) because cell recognition followed by membrane fusion between the viral envelope and the target cell is prerequisite for successful infection by envelope viruses. Here we demonstrate the competence of envelope-free lentivirus in establishing stable gene integration by germline transgenesis in mice at high efficiency, by intracytoplasmic viral injection (INVI) of envelope-free lentivirus into mouse zygotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.