ArticleEukaryotic cell2007
Further characterization of the signaling proteolysis step in the Aspergillus nidulans pH signal transduction pathway.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- Understanding the Role of pH Regulation and Neutralizing Agents in Organic Acid Production and Growth of Aspergillus oryzae.Biotechnology and bioengineering · 2026Article
- Co-Expression Network Analysis Suggests PacC Transcriptional Factor Involved inJournal of fungi (Basel, Switzerland) · 2025Article
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- Biophysical Characterization of the C-Terminal Tail ofACS omega · 2023Article
- State-of-the-Art Dermatophyte Infections: Epidemiology Aspects, Pathophysiology, and Resistance Mechanisms.Journal of fungi (Basel, Switzerland) · 2021Review
- Defining the transcriptional responses ofMicrobial genomics · 2020Article
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- Screening of Fungi for Potential Application of Self-Healing Concrete.Scientific reports · 2019Article
- The Beauveria bassiana Gas3 β-Glucanosyltransferase Contributes to Fungal Adaptation to Extreme Alkaline Conditions.Applied and environmental microbiology · 2018Article
- Functional diversity in the pH signaling pathway: an overview of the pathway regulation in Neurospora crassa.Current genetics · 2018Review
- PApplied and environmental microbiology · 2017Article
- Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds.Molecular microbiology · 2016Article
- Proteolytic activation of both components of the cation stress-responsive Slt pathway in Aspergillus nidulans.Molecular biology of the cell · 2016Article
- Ammonia activates pacC and patulin accumulation in an acidic environment during apple colonization by Penicillium expansum.Molecular plant pathology · 2016Article
- Molecular Components of the Neurospora crassa pH Signaling Pathway and Their Regulation by pH and the PAC-3 Transcription Factor.PloS one · 2016Article
- Refining the pH response in Aspergillus nidulans: a modulatory triad involving PacX, a novel zinc binuclear cluster protein.Molecular microbiology · 2015Article
- Aspergillus nidulans Ambient pH Signaling Does Not Require Endocytosis.Eukaryotic cell · 2015Article
- In Aspergillus nidulans the suppressors suaA and suaC code for release factors eRF1 and eRF3 and suaD codes for a glutamine tRNA.G3 (Bethesda, Md.) · 2014Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The Aspergillus nidulans pH-responsive transcription factor PacC is modulated by limited, two-step proteolysis. The first, pH-regulated cleavage occurs in the 24-residue highly conserved "signaling protease box" in response to the alkaline pH signal. This is transduced by the Pal signaling pathway, containing the predicted calpain-like cysteine protease and likely signaling protease, PalB. In this work, we carried out classical mutational analysis of the putative signaling protease PalB, and we describe 9 missense and 18 truncating loss-of-function (including null) mutations. Mutations in the region of and affecting directly the predicted catalytic cysteine strongly support the deduction that PalB is a cysteine protease. Truncating and missense mutations affecting the C terminus highlight the importance of this region. Analysis of three-hemagglutinin-tagged PalB in Western blots demonstrates that PalB levels are independent of pH and Pal signal transduction. We have followed the processing of MYC(3)-tagged PacC in Western blots. We show unequivocally that PalB is essential for signaling proteolysis and is definitely not the processing protease. In addition, we have replaced 15 residues of the signaling protease box of MYC(3)-tagged PacC (pacC900) with alanine. The majority of these substitutions are silent. Leu481Ala, Tyr493Ala, and Gln499Ala result in delayed PacC processing in response to shifting from acidic to alkaline medium, as determined by Western blot analysis. Leu498Ala reduces function much more markedly, as determined by plate tests and processing recalcitrance. Excepting Leu498, this demonstrates that PacC signaling proteolysis is largely independent of sequence in the cleavage region.
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Registered trials
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