ArticleBiophysical journal2007
Protein hydrophobic collapse and early folding steps observed in a microfluidic mixer.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
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- Theoretical Study of the Influence of K20N Glycosylation on the Dynamic Behavior of Im7 Protein.Molecules (Basel, Switzerland) · 2025Article
- A Tandem-Affinity Purification Method for Identification of Primary Intracellular Drug-Binding Proteins.ACS chemical biology · 2024Article
- Protein Design: From the Aspect of Water Solubility and Stability.Chemical reviews · 2022Review
- Advances in Mixer Design and Detection Methods for Kinetics Studies of Macromolecular Folding and Binding on the Microsecond Time Scale.Molecules (Basel, Switzerland) · 2022Article
- Complex Folding Landscape of Apomyoglobin at Acidic pH Revealed by Ultrafast Kinetic Analysis of Core Mutants.The journal of physical chemistry. B · 2018Article
- Article
- Specific and intrinsic sequence patterns extracted by deep learning from intra-protein binding and non-binding peptide fragments.Scientific reports · 2017Article
- Real-time monitoring of hydrophobic aggregation reveals a critical role of cooperativity in hydrophobic effect.Nature communications · 2017Article
- Translation and folding of single proteins in real time.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Application of conventional molecular dynamics simulation in evaluating the stability of apomyoglobin in urea solution.Scientific reports · 2017Article
- Protein unfolding mechanisms and their effects on folding experiments.F1000Research · 2017Review
- How cooperative are protein folding and unfolding transitions?Protein science : a publication of the Protein Society · 2016Review
- When fast is better: protein folding fundamentals and mechanisms from ultrafast approaches.The Biochemical journal · 2016Review
- Microfluidic chips with multi-junctions: an advanced tool in recovering proteins from inclusion bodies.Bioengineered · 2015Article
- Complex pathways in folding of protein G explored by simulation and experiment.Biophysical journal · 2014Article
- Fast protein folding kinetics.Quarterly reviews of biophysics · 2014Review
- Microsecond barrier-limited chain collapse observed by time-resolved FRET and SAXS.Journal of molecular biology · 2014Article
- Refolding techniques for recovering biologically active recombinant proteins from inclusion bodies.Biomolecules · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate that the sub-millisecond protein folding process referred to as "collapse" actually consists of at least two separate processes. We observe the UV fluorescence spectrum from naturally occurring tryptophans in three well-studied proteins, cytochrome c, apomyoglobin, and lysozyme, as a function of time in a microfluidic mixer with a dead time of approximately 20 mus. Single value decomposition of the time-dependent spectra reveal two separate processes: 1), a spectral shift which occurs within the mixing time; and 2), a fluorescence decay occurring between approximately 100 and 300 micros. We attribute the first process to hydrophobic collapse and the second process to the formation of the first native tertiary contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.