ArticleBiophysical journal2007
Scattering of exciting light by live cells in fluorescence confocal imaging: phototoxic effects and relevance for FRAP studies.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 87 citations in OpenAlex.
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- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- Dissection of DNA damage and repair pathways in live cells by femtosecond laser microirradiation and free-electron modeling.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell quantification in digital contrast microscopy images with convolutional neural networks algorithm.Scientific reports · 2023Article
- Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.Biomedical optics express · 2021Article
- Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.Cell chemical biology · 2020Article
- Between life and death: strategies to reduce phototoxicity in super-resolution microscopy.Journal of physics D: Applied physics · 2020Review
- Imaging Platelet Processes and Function-Current and Emerging Approaches for ImagingFrontiers in immunology · 2020Review
- Quantifying Single mRNA Translation Kinetics in Living Cells.Cold Spring Harbor perspectives in biology · 2018Review
- Aqueous mounting media increasing tissue translucence improve image quality in Structured Illumination Microscopy of thick biological specimen.Scientific reports · 2018Article
- Structured illumination to spatially map chromatin motions.Journal of biomedical optics · 2018Article
- Multimodal Light Microscopy Approaches to Reveal Structural and Functional Properties of Promyelocytic Leukemia Nuclear Bodies.Frontiers in oncology · 2018Review
- Direct Quantification of Solute Diffusivity in Agarose and Articular Cartilage Using Correlation Spectroscopy.Annals of biomedical engineering · 2017Article
- Mathematical imaging methods for mitosis analysis in live-cell phase contrast microscopy.Methods (San Diego, Calif.) · 2017Article
- Melanoma cells undergo aggressive coalescence in a 3D Matrigel model that is repressed by anti-CD44.PloS one · 2017Article
- Altered Cellular Homeostasis in Murine MPS I Fibroblasts: Evidence of Cell-Specific Physiopathology.JIMD reports · 2017Article
- Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2016Article
- Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking.The Journal of cell biology · 2015Article
- Tracking the activity-dependent diffusion of synaptic proteins using restricted photoconversion of Dendra2.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
As exciting light in a scanning confocal microscope encounters a cell and its subcellular components, it is refracted and scattered. A question arises as to what proportion of the exciting light is scattered by subcellular structures and whether cells in the vicinity of the imaged area, i.e., cells that are not directly illuminated by the laser beam, can be affected by either an exposure to scattered light and ensuing phototoxic reactions, or by the products of photoactivated reactions diffusing out of the directly illuminated area. We have designed a technique, which allows us to detect subtle cell photodamage and estimate the extent and range of phototoxic effects inflicted by interaction between scattered exciting light and fluorescent probes in the vicinity of the illuminated area. The technique is based on detecting an increased influx of acridine orange into photodamaged cells, which is manifested by a change of color. We demonstrate that phototoxic effects can be exerted not only on the illuminated cell, but also on fluorescently labeled neighboring cells. The damage inflicted on neighbors is due to exposure to light scattered by the imaged (i.e., directly illuminated) cell, but not phototoxic products diffusing out of the directly illuminated area. When light encounters a cell nucleus, scattering is so intense that photodamage can be inflicted even on fluorescently labeled cells located within a radius of approximately 90 microm, i.e., several cell diameters away. This range of scattering is comparable with that caused by the glass bead resting on a coverslip (up to 120 microm). The intense scattering of exciting light imposes limits on FRAP, FLIP, and other techniques employing high intensity laser beams.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.