ReviewTransgenic research2007
Adopting the good reFLEXes when generating conditional alterations in the mouse genome.
Review in Transgenic research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Transsynaptic linking of calcium channels and postsynaptic receptors at a dyadic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Activity induced GEM-4/Copine expression inhibits gap junctions and promotes thermosensory plasticity.bioRxiv : the preprint server for biology · 2026Article
- Engineering Base Changes and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.Current protocols · 2025Article
- Post-synaptic GABACell reports · 2023Article
- Highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence.Cell reports methods · 2023Article
- JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm.Nature communications · 2022Article
- CASK and FARP localize two classes of post-synaptic ACh receptors thereby promoting cholinergic transmission.PLoS genetics · 2022Article
- Shank promotes action potential repolarization by recruiting BK channels to calcium microdomains.eLife · 2022Article
- Interleukin-7 receptor α mutational activation can initiate precursor B-cell acute lymphoblastic leukemia.Nature communications · 2021Article
- Characterization of kindled VGAT-Cre mice as a new animal model of temporal lobe epilepsy.Epilepsia · 2020Article
- Erythrocyte-derived microvesicles induce arterial spasms in JAK2V617F myeloproliferative neoplasm.The Journal of clinical investigation · 2020Article
- Testing the Cre-mediated genetic switch for the generation of conditional knock-in mice.PloS one · 2019Article
- The stable actin core of mechanosensory stereocilia features continuous turnover of actin cross-linkers.Molecular biology of the cell · 2018Article
- Dissection of zebrafisheLife · 2017Article
- Absence of neurological abnormalities in mice homozygous for the Polr3a G672E hypomyelinating leukodystrophy mutation.Molecular brain · 2017Article
- Length regulation of mechanosensitive stereocilia depends on very slow actin dynamics and filament-severing proteins.Nature communications · 2015Article
- The role of PTPN22 risk variant in the development of autoimmunity: finding common ground between mouse and human.Journal of immunology (Baltimore, Md. : 1950) · 2015Review
- A conditional system to specifically link disruption of protein-coding function with reporter expression in mice.Cell reports · 2014Article
- The SLC6 transporters: perspectives on structure, functions, regulation, and models for transporter dysfunction.Pflugers Archiv : European journal of physiology · 2014Review
- Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.Nucleic acids research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Major advances have been made in the use of the Cre/loxP system for conditional gene targeting in the mouse. By combining the ability of Cre recombinase to invert or excise a DNA fragment, depending upon the orientation of the flanking loxP sites, and the use of wild-type loxP and variant lox511 sites, we devised an efficient and reliable Cre-mediated genetic switch, called FLEX, through which expression of a given gene can be turned off, while expression of another one can be simultaneously turned on. We discuss how this innovative, flexible and powerful approach, which virtually adapts to any kind of site-specific recombinase (e.g., Cre and Flp recombinases), can be used to easily generate, even at high throughput and genome wide scale, many genetic modifications in a conditional manner, including those which were considered as difficult or impossible to achieve.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.