ArticleNucleic acids research2007
Concentration-dependent organization of DNA by the dinoflagellate histone-like protein HCc3.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Nucleosome Assembly Protein 1 in Dinoflagellate Cells Without Architectural Nucleosomes.International journal of molecular sciences · 2026Article
- Dinochromosome Heterotermini with Telosomal Anchorages.International journal of molecular sciences · 2024Article
- Review
- Genome-wide distribution of 5-hydroxymethyluracil and chromatin accessibility in the Breviolum minutum genome.Genome biology · 2024Article
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Genome-wide distribution of 5-hydroxymethyluracil and chromatin accessibility in thebioRxiv : the preprint server for biology · 2023Article
- Genetic and spatial organization of the unusual chromosomes of the dinoflagellate Symbiodinium microadriaticum.Nature genetics · 2021Article
- The DNA-binding protein HTa fromeLife · 2019Article
- Omics Analysis for Dinoflagellates Biology Research.Microorganisms · 2019Review
- The Biochemistry and Evolution of the Dinoflagellate Nucleus.Microorganisms · 2019Review
- Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net.Scientific reports · 2019Article
- Distinctive Nuclear Features of Dinoflagellates with A Particular Focus on Histone and Histone-Replacement Proteins.Microorganisms · 2018Review
- Translation and Translational Control in Dinoflagellates.Microorganisms · 2018Review
- Major transitions in dinoflagellate evolution unveiled by phylotranscriptomics.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Diversity and Divergence of Dinoflagellate Histone Proteins.G3 (Bethesda, Md.) · 2015Article
- A histone-like protein induces plasmid DNA to form liquid crystals in vitro and gene compaction in vivo.International journal of molecular sciences · 2013Article
- Article
- Exotic mitotic mechanisms.Open biology · 2012Review
- A full suite of histone and histone modifying genes are transcribed in the dinoflagellate Lingulodinium.PloS one · 2012Article
- Birefringence and DNA condensation of liquid crystalline chromosomes.Eukaryotic cell · 2010Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The liquid crystalline chromosomes of dinoflagellates are the alternative to the nucleosome-based organization of chromosomes in the eukaryotes. These nucleosome-less chromosomes have to devise novel ways to maintain active parts of the genome. The dinoflagellate histone-like protein HCc3 has significant sequence identity with the bacterial DNA-binding protein HU. HCc3 also has a secondary structure resembling HU in silico. We have examined HCc3 in its recombinant form. Experiments on DNA-cellulose revealed its DNA-binding activity is on the C-terminal domain. The N-terminal domain is responsible for intermolecular oligomerization as demonstrated by cross-linking studies. However, HCc3 could not complement Escherichia coli HU-deficient mutants, suggesting functional differences. In ligation assays, HCc3-induced DNA concatenation but not ring closure as the DNA-bending HU does. The basic HCc3 was an efficient DNA condensing agent, but it did not behave like an ordinary polycationic compound. HCc3 also induced specific structures with DNA in a concentration-dependent manner, as demonstrated by atomic force microscopy (AFM). At moderate concentration of HCc3, DNA bridging and bundling were observed; at high concentrations, the complexes were even more condensed. These results are consistent with a biophysical role for HCc3 in maintaining extended DNA loops at the periphery of liquid crystalline chromosomes.
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