ArticleMolecular and cellular biochemistry2007
Purification and kinetic properties of glutathione reductase from bovine liver.
Article in Molecular and cellular biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Effect of Nitrosative Stress on Biofilm Formation and Upregulation of 2,3-Butanediol Dehydrogenase in Saccharomyces Cerevisiae.Current microbiology · 2025Article
- NADPH Dynamics: Linking Insulin Resistance and β-Cells Ferroptosis in Diabetes Mellitus.International journal of molecular sciences · 2023Review
- Purification and characterisation of glutathione reductase from scorpionfishJournal of enzyme inhibition and medicinal chemistry · 2023Article
- Exploring multifunctional antioxidants as potential agents for management of neurological disorders.Environmental science and pollution research international · 2022Review
- The possible importance of the antioxidants and oxidative stress metabolism in the emerging monkeypox disease: An opinion paper.Frontiers in public health · 2022Article
- Glutathione in Brain: Overview of Its Conformations, Functions, Biochemical Characteristics, Quantitation and Potential Therapeutic Role in Brain Disorders.Neurochemical research · 2020Review
- Low concentrations of clarithromycin upregulate cellular antioxidant enzymes and phosphorylation of extracellular signal-regulated kinase in human small airway epithelial cells.Journal of pharmaceutical health care and sciences · 2018Article
- Curious Cases of the Enzymes.Journal of medical biochemistry · 2015Review
- Evolution of Enzyme Kinetic Mechanisms.Journal of molecular evolution · 2015Review
- Age-dependent changes of the antioxidant system in rat livers are accompanied by altered MAPK activation and a decline in motor signaling.EXCLI journal · 2015Article
- A biophysically based mathematical model for the catalytic mechanism of glutathione reductase.Free radical biology & medicine · 2013Article
- Integration of a precolumn fluorogenic reaction, separation, and detection of reduced glutathione.Analytical chemistry · 2010Article
- A comparative study with colchicine on glutathione reductase.The protein journal · 2010Article
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2 authors.
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Abstract
Glutathione reductase (GR, NADPH: oxidized glutathione oxidoreductase, EC 1.6.4.2) catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using NADPH as reducing cofactor. The aim of the present work was to purify and characterize GR from bovine liver. GR was purified using 2', 5' ADP-Sepharose 4B and DEAE-Sepharose Fast Flow columns. The enzyme has been purified 5456-fold and with a yield of 38.4%. The molecular and catalytic properties of bovine liver GR have been studied. Optimum temperature and pH was found to be 50 degrees C and 7, respectively. The activation energy of the reaction catalyzed by the enzyme was 9.065 kcal/mole. The molecular weight of the enzyme was found to be 55 kDa by SDS-PAGE. Kinetic characterization of bovine liver GR was also investigated, Km(NADPH) 0.063 +/- 0.008 mM and Km(GSSG) 0.154 +/- 0.015 mM were determined. It is accepted that parallel lines observed in these double reciprocal plots obeys Ping Pong mechanism and we have showed this in our steady state study. According to our results of statistical analysis, the Ping Pong mechanism is a suitable model since the loss function is less than the other mechanisms. However, competitive inhibition by a product could be accepted in sequential mechanisms but not in a Ping Pong mechanism. In this study, kinetic data are consistent with a branching reaction mechanism previously proposed for GR from other sources by other studies.
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